Al-Madhagi Wafa M, Mohd Hashim Najihah, Awad Ali Nasser A, Alhadi Abeer A, Abdul Halim Siti Nadiah, Othman Rozana
Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Center of Natural Products Research and Drug Discovery (CENAR), University of Malaya, Kuala Lumpur, Malaysia.
PeerJ. 2018 Jun 7;6:e4839. doi: 10.7717/peerj.4839. eCollection 2018.
belongs to the family of Piperaceae. It has different uses in folk medicine and contains rare compounds that have led to increased interest in this genus. (Jacq.) Kunth is used as an injury disinfectant by Yemeni people. In addition, the majority of Yemen's population still depend on the traditional remedy for serious diseases such as cancer, inflammation and infection. Currently, there is a deficiency of scientific evidence with regards to the medicinal plants from Yemen. Therefore, this study was performed to assess the chemical profile and antioxidant and cytotoxic activities of
Chemical profiling of was carried out using gas chromatography mass spectrometry (GCMS) followed by isolation of bioactive compounds by column chromatography. DPPH• and FRAP assays were used to evaluate antioxidant activity and the MTT assay was performed to estimate the cytotoxicity activity against three cancer cell lines, namely MCF-7, HL-60 and WEHI-3, and three normal cell lines, MCF10A, WRL-68 and HDFa.
X-ray crystallographic data for peperomin A is reported for the first time here and '-diphenethyloxamide was isolated for the first time from . Methanol and dichloromethane extracts showed high radical scavenging activity with an IC of 36.81 ± 0.09 µg/mL, followed by the dichloromethane extract at 61.78 ± 0.02 µg/mL, whereas the weak ferric reducing activity of extracts ranging from 162.2 ± 0.80 to 381.5 ± 1.31 µg/mL were recorded. In addition, petroleum ether crude extract exhibited the highest cytotoxic activity against all the tested cancer cell lines with IC values of 9.54 ± 0.30, 4.30 ± 0.90 and 5.39 ± 0.34 µg/mL, respectively. Peperomin A and the isolated mixture of phytosterol (stigmasterol and β-sitosterol) exhibited cytotoxic activity against MCF-7 and WE-HI cell lines with an IC of (5.58 ± 0.47, 4.62 ± 0.03 µg/mL) and (8.94 ± 0.05, 9.84 ± 0.61 µg/mL), respectively, compared to a standard drug, taxol, that has IC values of 3.56 ± 0.34 and 1.90 ± 0.9 µg/mL, respectively.
The activities of extracts and isolated compounds recorded in this study underlines the potential that makes this plant a valuable source for further study on anticancer and antioxidant activities.
属于胡椒科。它在民间医学中有不同用途，且含有稀有化合物，这使得人们对该属植物的兴趣增加。（雅克）昆特被也门人用作伤口消毒剂。此外，也门的大多数人口仍然依赖传统疗法来治疗癌症、炎症和感染等严重疾病。目前，关于也门药用植物的科学证据不足。因此，本研究旨在评估[植物名称未给出]的化学特征、抗氧化和细胞毒性活性。
使用气相色谱 - 质谱联用（GCMS）对[植物名称未给出]进行化学分析，随后通过柱色谱法分离生物活性化合物。采用DPPH•和FRAP测定法评估抗氧化活性，并进行MTT测定以评估对三种癌细胞系（即MCF - 7、HL - 60和WEHI - 3）以及三种正常细胞系（MCF10A、WRL - 68和HDFa）的细胞毒性活性。
首次在此报告了胡椒素A的X射线晶体学数据，并且首次从[植物名称未给出]中分离出了N - N'-二苯乙酰胺。甲醇和二氯甲烷提取物表现出高自由基清除活性，IC50为36.81±0.09μg/mL，其次是二氯甲烷提取物，IC50为61.78±0.02μg/mL，而提取物的弱铁还原活性范围为162.2±0.80至381.5±1.31μg/mL。此外，石油醚粗提物对所有测试的癌细胞系表现出最高的细胞毒性活性，IC50值分别为9.54±0.30、4.30±0.90和5.39±0.34μg/mL。胡椒素A和分离出的植物甾醇混合物（豆甾醇和β - 谷甾醇）对MCF - 7和WE - HI细胞系表现出细胞毒性活性，IC50分别为（5.58±0.47，4.62±0.03μg/mL）和（8.94±0.05，9.84±0.61μg/mL），相比之下，标准药物紫杉醇的IC50值分别为3.56±0.34和1.90±0.9μg/mL。
本研究中记录的[植物名称未给出]提取物和分离化合物的活性强调了该植物作为进一步研究抗癌和抗氧化活性的宝贵来源的潜力。
