Department of Dermatology, Tehran University of Medical Sciences, Razi Hospital, Tehran, Iran.
Department of Pathology, Tehran University of Medical Sciences, Razi Hospital, Tehran, Iran.
Trop Biomed. 2019 Dec 1;36(4):1061-1070.
There are little information about Th17 cells and cutaneous Leishmaniasis (CL), due to an important effect of Th17 cells on immune response, it is worth to explore the role of Th17 on CL. The purpose of this study was to assess Th17 population in patients with acute vs. chronic CL lesions in comparison with skin samples collected from healthy volunteers in an endemic region of Old World CL. A total of 49 patients with clinical manifestations of chronic (n=16) and acute (n=33) CL lesions were recruited. The clinical diagnosis of CL was confirmed by direct smear or PCR. Biopsy specimens from prelesional skin of non-infectious lesions of 30 healthy individuals were used as control. Tissue sections of 3µm thickness were prepared and used for immunohistochemistry (IHC) analysis with primary antibody specific for Th17 associated antigen (CD161). For IHC, Envision+ (DakoCytomation) system was used and developed by using diaminobenzidine (DakoCytomation). The mean age of 33 patients with acute CL and the mean age of 16 patients with chronic CL were accordingly 45.24±16.43 and 33.56±15.87. In acute and chronic CL the mean (±standard deviation) and median (±interquartile range) were accordingly 2.92±2.21, 2.56±2.9 and 2.1±1.99, 1.54±2.81. In healthy controls the mean (±standard deviation) and median (±interquartile range) were 0.72±0.41 and 0.61±0.58 respectively. With pairwise comparison of acute, chronic and control groups, there were significant difference between acute and control (P value < 0.001), chronic and control (P value = 0.043). The results showed that there was an increasing cellular response of Th17 in both acute and chronic CL patients. Th17 was significantly higher in patients with acute and chronic CL lesions in comparison with healthy control group. However, there was no significant difference between acute and chronic infection concerning to Th17 cells.
有关 Th17 细胞和皮肤利什曼病 (CL) 的信息很少，由于 Th17 细胞对免疫反应有重要影响，因此值得探讨 Th17 在 CL 中的作用。本研究的目的是评估急性与慢性 CL 病变患者的 Th17 群体，并与旧世界 CL 流行地区健康志愿者的皮肤样本进行比较。共招募了 49 例具有慢性 (n=16) 和急性 (n=33) CL 病变临床表现的患者。CL 的临床诊断通过直接涂片或 PCR 确认。从 30 名健康个体的非感染性病变的预病变皮肤中采集活检标本作为对照。制备 3µm 厚的组织切片，并使用针对 Th17 相关抗原 (CD161) 的特异性一抗进行免疫组织化学 (IHC) 分析。对于 IHC，使用 Envision+(DakoCytomation) 系统，并使用二氨基联苯胺 (DakoCytomation) 进行开发。33 例急性 CL 患者的平均年龄和 16 例慢性 CL 患者的平均年龄分别为 45.24±16.43 和 33.56±15.87。在急性和慢性 CL 中，平均值 (±标准差) 和中位数 (±四分位距) 分别为 2.92±2.21、2.56±2.9 和 2.1±1.99、1.54±2.81。在健康对照组中，平均值 (±标准差) 和中位数 (±四分位距) 分别为 0.72±0.41 和 0.61±0.58。通过急性、慢性和对照组之间的两两比较，急性和对照组之间存在显著差异 (P 值<0.001)，慢性和对照组之间存在显著差异 (P 值=0.043)。结果表明，急性和慢性 CL 患者的 Th17 细胞均存在细胞反应增加。与健康对照组相比，急性和慢性 CL 患者的 Th17 明显更高。然而，急性和慢性感染之间的 Th17 细胞没有显著差异。
