Mortazavi H, Sadeghipour P, Taslimi Y, Habibzadeh S, Zali F, Zahedifard F, Rahmati J, Kamyab K, Ghandi N, Zamanian A, Reza Tohidinik H, Muller I, Kropf P, Rafati S
Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran.
J Eur Acad Dermatol Venereol. 2016 Dec;30(12):2118-2121. doi: 10.1111/jdv.13838. Epub 2016 Jul 21.
Cutaneous leishmaniasis (CL) in Iran is mainly caused by Leishmania major (L. major) and L. tropica. Arginase mediated L-arginine metabolism is an important issue in Leishmania parasite propagation. Arginase activity in human CL due to L. major and L. tropica have not been studied up to now.
We aimed to compare the clinical and laboratory aspects of acute and chronic CL, focussing on arginase activity.
In this case-control study, 30 patients with acute CL (duration ≤ 1 year), 13 patients with chronic CL (duration ≥ 2 year) and 11 healthy controls were recruited. Arginase activity was measured in skin biopsies of lesions, peripheral blood polymorphonuclear cells (PMNs), peripheral blood mononuclear cells (PBMCs) and plasma by standard methods.
The median of arginase activity in the acute lesions was higher than in chronic samples and significantly higher than in healthy controls (P = 0.008). PMNs of both acute and chronic patients showed higher levels of arginase activity as compared to the levels in PBMCs and plasma. The median of arginase activity in the PMNs of patients with chronic CL was higher than that of patients with acute CL and significantly higher than that of the healthy controls (P = 0.010).
The level of arginase activity in lesions of patients with acute and chronic CL was higher than the skin of healthy controls. The highest level of arginase activity was observed in PMNs from patients with chronic CL. This suggests that the high level of arginase activity in PMNs of patients with chronic CL may contribute to the chronicity.
伊朗的皮肤利什曼病（CL）主要由硕大利什曼原虫（L. major）和热带利什曼原虫（L. tropica）引起。精氨酸酶介导的L-精氨酸代谢是利什曼原虫寄生虫繁殖中的一个重要问题。迄今为止，尚未对由硕大利什曼原虫和热带利什曼原虫引起的人类皮肤利什曼病中的精氨酸酶活性进行研究。
我们旨在比较急性和慢性皮肤利什曼病的临床和实验室方面，重点关注精氨酸酶活性。
在这项病例对照研究中，招募了30例急性皮肤利什曼病患者（病程≤1年）、13例慢性皮肤利什曼病患者（病程≥2年）和11名健康对照者。通过标准方法测量病变皮肤活检组织、外周血多形核细胞（PMN）、外周血单核细胞（PBMC）和血浆中的精氨酸酶活性。
急性病变中精氨酸酶活性的中位数高于慢性样本，且显著高于健康对照（P = 0.008）。与PBMC和血浆中的水平相比，急性和慢性患者的PMN均显示出较高水平的精氨酸酶活性。慢性皮肤利什曼病患者PMN中精氨酸酶活性的中位数高于急性皮肤利什曼病患者，且显著高于健康对照（P = 0.010）。
急性和慢性皮肤利什曼病患者病变部位的精氨酸酶活性水平高于健康对照者的皮肤。在慢性皮肤利什曼病患者的PMN中观察到最高水平的精氨酸酶活性。这表明慢性皮肤利什曼病患者PMN中高水平的精氨酸酶活性可能导致疾病的慢性化。
