Tembo Maiwase, Sauer Monica L, Wisner Bennett W, Beleny David O, Napolitano Marc A, Carlson Anne E
Department of Biological Sciences, University of Pittsburgh.
MicroPubl Biol. 2021 Feb 9;2021. doi: 10.17912/micropub.biology.000365.
Fertilization of an egg by multiple sperm presents one of the earliest and most prevalent obstacles to successful reproduction. Eggs employ multiple mechanisms to prevent sperm entry into the nascent zygote. The fast block to polyspermy uses a depolarization to inhibit sperm entry. For some external fertilizers, fertilization and the fast block require actin polymerization. Here we explored whether the fast block to polyspermy in the external fertilizer, , requires actin polymerization. Inseminating in the presence of inhibitor cytochalasin B, here we demonstrate that actin polymerization is not required for the fast block to polyspermy in .
多个精子使卵子受精是成功繁殖最早且最普遍的障碍之一。卵子采用多种机制来阻止精子进入新形成的受精卵。多精受精的快速阻断利用去极化来抑制精子进入。对于一些体外受精的生物，受精和快速阻断需要肌动蛋白聚合。在这里，我们探究了体外受精生物中多精受精的快速阻断是否需要肌动蛋白聚合。在存在抑制剂细胞松弛素B的情况下进行授精，我们在此证明，对于[具体生物名称未给出]中多精受精的快速阻断，肌动蛋白聚合并非必需。
