Ma Mingbiao, Yuan Mei, Li Ming, Li Xiaojuan, Huang Hailin, Wang Haiping, Li Jue, Du Tingyi, Huang Rongwei
Department of Clinical Laboratory, Kunming Children's Hospital, Kunming, 650500, China.
Yunnan Key Laboratory of Children's Major Disease Research, Kunming, 650500, China.
Curr Microbiol. 2021 Mar;78(3):954-960. doi: 10.1007/s00284-021-02365-4. Epub 2021 Feb 18.
Streptococcus pneumoniae (S. pneumoniae) is the main conditional pathogen of acute respiratory infection in infants, children, and older adults worldwide. It was great significant to identify the epidemic characteristics of serotypes and antibiotic susceptibility for the prevention and treatment of S. pneumoniae diseases. This research assessed the serotype distribution and the minimum inhibitory concentrations (MICs) of S. pneumoniae isolated from pediatric patients to provide information on the epidemiology and antibiotic resistance of S. pneumoniae in Kunming, China. A total of 140 S. pneumoniae isolates were collected from pediatric patients at Kunming Children's Hospital from January 2016 to October 2017. Serotype identification was done by Quellung reaction and multiplex polymerase chain reaction. MICs were determined by E-test. 140 isolates distributed in 13 types of serotypes. The top-three prevalent serotypes were 19F, 19A, and 6B. The immunization coverage rate of 13-valent pneumococcal conjugate vaccine (PCV) was relatively higher and should be introduced into the vaccination program in the region. MIC of penicillin, ceftriaxone, and levofloxacin was 1 μg/mL. MIC for meropenem and vancomycin was 0.38 μg/mL. MIC of penicillin, ceftriaxone, and levofloxacin was 1.5 μg/mL and that of meropenem and vancomycin was 0.5 μg/mL. The MIC of erythromycin was > 256 μg/mL. In summary, S. pneumoniae had low resistance rates to penicillin, ceftriaxone, levofloxacin, vancomycin, and meropenem, and these antibiotics could be the first-line agents for children with pneumococcal infections in Kunming.
肺炎链球菌是全球婴幼儿、儿童及老年人急性呼吸道感染的主要条件致病菌。明确血清型流行特征及抗生素敏感性对于肺炎链球菌疾病的防治具有重要意义。本研究评估了从儿童患者中分离出的肺炎链球菌的血清型分布及最低抑菌浓度（MIC），以提供中国昆明地区肺炎链球菌的流行病学及抗生素耐药性信息。2016年1月至2017年10月期间，从昆明儿童医院的儿童患者中收集了140株肺炎链球菌分离株。通过荚膜肿胀反应和多重聚合酶链反应进行血清型鉴定。采用E-test法测定MIC。140株分离株分布于13种血清型。前三流行血清型为19F、19A和6B。13价肺炎球菌结合疫苗（PCV）的免疫覆盖率相对较高，应引入该地区的疫苗接种计划。青霉素、头孢曲松和左氧氟沙星的MIC为1μg/mL。美罗培南和万古霉素的MIC为0.38μg/mL。青霉素、头孢曲松和左氧氟沙星的MIC为1.5μg/mL，美罗培南和万古霉素的MIC为0.5μg/mL。红霉素的MIC>256μg/mL。综上所述，肺炎链球菌对青霉素、头孢曲松、左氧氟沙星、万古霉素和美罗培南的耐药率较低，这些抗生素可作为昆明地区肺炎球菌感染儿童的一线用药。
