Department of Forest Botany, Poznań University of Life Sciences, Poznań, Poland.
Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland.
PLoS One. 2021 Feb 18;16(2):e0243993. doi: 10.1371/journal.pone.0243993. eCollection 2021.
The pollen morphology was studied of 24 Salix species native to Poland, which represented two subgenera, 17 sections and five subsections occurring in Poland. The aim of this study was to discover the taxonomical usefulness of the pollen features under analysis, and to investigate the ranges of their interspecific variability. In total, 720 pollen grains were studied. They were analysed with respect to seven quantitative features (length of the polar axis - P, equatorial diameter - E, length of the ectoaperture - Le, exine thickness - Ex, and P/E, Ex/P and Le/P ratios) and the following qualitative ones: pollen outline and exine ornamentation. The most important features were exine ornamentation (muri, lumina and margo) characters. The pollen features should be treated as auxiliary because they allowed to distinguish eight individual Salix species, and five groups of species. Statistical analysis of the studied traits indicated a high variability among the tested species. The most variable biometric features were P, E and Le, while lower variability occurred in P/E, Le/P and d/E.
对波兰原产的 24 种柳属植物的花粉形态进行了研究，这些植物代表了在波兰出现的两个亚属、17 个组和 5 个亚组。本研究的目的是发现所分析的花粉特征在分类学上的有用性，并研究其种间变异性的范围。总共研究了 720 个花粉粒。从七个定量特征（极轴长度 - P、赤道直径 - E、外孔长度 - Le、外壁厚度 - Ex 以及 P/E、Ex/P 和 Le/P 比）和以下定性特征（花粉轮廓和外壁纹饰）方面对其进行了分析。外壁纹饰（壁突、腔和边缘）特征是最重要的特征。花粉特征应作为辅助特征，因为它们可以区分 8 种柳属植物和 5 个种的组。对所研究特征的统计分析表明，测试物种之间具有高度的变异性。最具变异性的生物计量特征是 P、E 和 Le，而 P/E、Le/P 和 d/E 的变异性较低。
