Department of Plant Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.
Department of Botany, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan.
Microsc Res Tech. 2021 Apr;84(4):608-617. doi: 10.1002/jemt.23619. Epub 2020 Oct 16.
The present study aimed to investigate the palyno-morphological features of species of family Vitaceae from Pakistan. A total of nine species, belonging to four genera were collected, pressed, identified, and then analyzed microscopically. Both quantitative and qualitative characters of the pollen grains were recorded including polar and equatorial diameter, P/E ratio, number of colpi and pores, exine thickness and shapes of the pollen in both polar and equatorial view, and exine sculpturing using Leica microscope fitted with camera Meiji Infinity 1 and then analyzed statistically using software IBM SPSS Statistics 20. The results of the present study demonstrated the variations in polar and equatorial diameter, exine thickness, P/E ratio, pollen shape, and exine sculpturing of the studied species and highlighted the significance of pollen morphology as an identification tool. The present study may contribute to better understand the classification at genus level, which will support the future phylogenetic characterization of the family.
本研究旨在探讨巴基斯坦葡萄科植物的花粉形态特征。共采集、压制、鉴定了 9 种，分属 4 属，然后进行了微观分析。记录了花粉粒的定量和定性特征，包括极轴和赤道直径、P/E 比、裂缝和孔的数量、外壁厚度以及极面和赤道面的花粉形状，然后使用配备有 Meiji Infinity 1 相机的徕卡显微镜进行外壁纹饰分析，最后使用 IBM SPSS Statistics 20 软件进行统计分析。本研究结果表明，所研究物种的极轴和赤道直径、外壁厚度、P/E 比、花粉形状和外壁纹饰存在差异，并强调了花粉形态作为鉴定工具的重要性。本研究可能有助于更好地理解属级分类，这将支持该科未来的系统发育特征分析。
