Department of Biochemistry, Faculty of Pharmacy, Sivas Cumhuriyet University, 58140 Sivas, Turkiye.
Department of Biochemistry, Faculty of Pharmacy, Sivas Cumhuriyet University, 58140 Sivas, Turkiye.
J Gynecol Obstet Hum Reprod. 2021 Apr;50(4):102092. doi: 10.1016/j.jogoh.2021.102092. Epub 2021 Feb 15.
Endometriosis is defined by the presence of endometrial glands and stroma grow in areas outside the uterus. A simple blood test for endometriosis-specific biomarkers would offer a more timely accurate diagnosis of the disease and could lead to earlier treatment intervention. Alterations in microRNA (miRNA) levels in blood may reflect changes during normal physiologic processes and have been related to several pathologic conditions, including gynecologic diseases. In the present study, we aim to evaluate the level of serum miR-34a-5p and miR-200c from women with and without endometriosis, and to explore the potential of miRNAs as reliable non-invasive biomarkers in the diagnosis of endometriosis.
Expression levels of miRNAs were performed by quantitative real-time polymerase chain reaction (qRT-PCR). Serum cancer antigen 125 (CA-125) levels were analyzed by autoanalyzer.
miR-34a-5p expression levels were decreased and miR-200c expression levels were increased in the endometriosis patients compared to the control group. According to the areas under the ROC curve (AUC) values, miR-200c and miR-34a-5p may serve as biomarkers for the diagnosis of endometriosis. Serum miR-34a-5p and miR-200c had a sensitivity of 78.95 % and 100 % and a specificity of 49.12 % and 100 %, respectively, for the detection of endometriosis.
Serum miRNAs may provide a promising opportunity for diagnosis of endometriosis. Understanding the role of circulating miRNAs will serve a better comprehension of the systemic effects of endometriosis and offer options for new treatments. It is clear that more work is needed in this area.
子宫内膜异位症是指子宫内膜腺体和间质出现在子宫以外的部位生长。一种简单的检测子宫内膜异位症特异性生物标志物的血液检测方法可以更及时、更准确地诊断疾病，并可能导致更早的治疗干预。血液中 microRNA（miRNA）水平的改变可能反映了正常生理过程中的变化，并与包括妇科疾病在内的几种病理状况有关。在本研究中，我们旨在评估患有和不患有子宫内膜异位症的女性血清 miR-34a-5p 和 miR-200c 的水平，并探讨 miRNA 作为可靠的非侵入性生物标志物在子宫内膜异位症诊断中的潜力。
通过实时定量聚合酶链反应（qRT-PCR）检测 miRNA 的表达水平。通过自动分析仪分析血清癌抗原 125（CA-125）水平。
与对照组相比，子宫内膜异位症患者的 miR-34a-5p 表达水平降低，miR-200c 表达水平升高。根据 ROC 曲线下面积（AUC）值，miR-200c 和 miR-34a-5p 可作为子宫内膜异位症诊断的生物标志物。血清 miR-34a-5p 和 miR-200c 检测子宫内膜异位症的敏感性分别为 78.95%和 100%，特异性分别为 49.12%和 100%。
血清 miRNAs 可能为子宫内膜异位症的诊断提供有前途的机会。了解循环 miRNAs 的作用将有助于更好地理解子宫内膜异位症的全身效应，并为新的治疗方法提供选择。很明显，这一领域还需要做更多的工作。
