血浆 microRNA-200 家族表达与狼疮肾炎风险和疾病严重程度的相关性。

The correlation of plasma microRNA-200 family expressions with risk and disease severity of lupus nephritis.

机构信息

Department of TCM, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

出版信息

Eur Rev Med Pharmacol Sci. 2018 May;22(10):3118-3125. doi: 10.26355/eurrev_201805_15070.

DOI:10.26355/eurrev_201805_15070

PMID:29863256

Abstract

OBJECTIVE

To investigate whether plasma microRNA-200 family expressions could serve as diagnostic biomarkers in patients with lupus nephritis (LN), and their association with disease severity.

PATIENTS AND METHODS

101 adult LN patients and 100 adult health volunteers were recruited in our study. A blood sample was obtained from all participants, and total RNA was extracted from plasma. Real-time PCR was performed to evaluate the relative expression of microRNA (miRNA). SLE disease activity index (SLEDAI) score was calculated to evaluate the overall disease severity.

RESULTS

Plasma miR-200b-5p, miR-141-5p, and miR-200c-5p expressions were decreased in LN patients compared to that in health controls (HCs). Multivariate logistic regression model revealed that plasma miR-200b-5p, miR-141-5p, and miR-200c-5p expressions were independent protective factors for predicting LN states. Receiver operating curve (ROC) was conducted to assess the diagnostic value of miR-200b-5p, miR-141-5p, and miR-200c-5p, and they revealed good diagnostic value with the area under curve (AUC) of 0.748, 0.748, 0.723, respectively. When miR-200b-5p, miR-141-5p, and miR-200c-5p are combined each other, the AUC increased to 0.936, suggesting a great diagnostic value of LN. Also, plasma miR-141-5p was observed to be negatively associated with serum creatinine and SLEDAI score, and the inverse correlations were found of miR-200c-5p with SLEDAI score, as well as miR-200b-3p with proteinuria.

CONCLUSIONS

Circulating miR-200b-5p, miR-141-5p, and miR-200c-5p expressions could be served as novel and convincing diagnostic biomarkers for LN.

摘要

目的

探讨血浆微小 RNA-200 家族表达能否作为狼疮肾炎（LN）患者的诊断生物标志物，及其与疾病严重程度的关系。

方法

本研究纳入 101 例成年 LN 患者和 100 例成年健康志愿者。所有参与者均采集血样，提取血浆总 RNA。采用实时 PCR 评估微小 RNA（miRNA）的相对表达。计算系统性红斑狼疮疾病活动指数（SLEDAI）评分，以评估整体疾病严重程度。

结果

与健康对照者（HCs）相比，LN 患者的血浆 miR-200b-5p、miR-141-5p 和 miR-200c-5p 表达降低。多变量逻辑回归模型显示，血浆 miR-200b-5p、miR-141-5p 和 miR-200c-5p 表达是预测 LN 状态的独立保护因素。绘制受试者工作特征曲线（ROC）评估 miR-200b-5p、miR-141-5p 和 miR-200c-5p 的诊断价值，曲线下面积（AUC）分别为 0.748、0.748、0.723，提示具有良好的诊断价值。当 miR-200b-5p、miR-141-5p 和 miR-200c-5p 相互结合时，AUC 增加至 0.936，提示对 LN 具有较高的诊断价值。此外，还观察到血浆 miR-141-5p 与血清肌酐和 SLEDAI 评分呈负相关，miR-200c-5p 与 SLEDAI 评分呈负相关，miR-200b-3p 与蛋白尿呈负相关。