Abdullah Saghir Ahmad, Imran Rashid, Kamran Ashraf, Wasim Shehzad, Matiullah Khan, Kashif Hussain, Shahid Hussain Farooqi, Amjad Khan, Muhammad Luqman Sohail
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore.
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore.
Trop Biomed. 2018 Sep 1;35(3):593-603.
Canine babesiosis is an important tick-borne protozoal disease of dogs that poses major health problem worldwide. Farm dogs in rural areas are the companion animals, that not only watch the livestock herds but also guard the house of the owners. Each farmer keeps his companion dog to get all the services. In our study, a total of 450 blood samples of farm dogs from three different ecological zones (Southern, Central and Northern regions of the province; Punjab) of Pakistan, were collected to examine through microscopy and PCR. Examination of thin blood smears revealed an overall prevalence of 12.8% (58/450) of canine babesisal parasites. However, PCR analysis revealed 46.8% (211/450) and 7.3% (33/450) samples positive for B. gibsoni and B. vogeli, respectively. The amplicons of 671 bp and 590 bp were amplified for the detection of B. gibsoni and B. vogeli, respectively through PCR. The results of multivariate analysis showed that the occurrence of canine babesiosis is higher in the Central Punjab and younger age of the dogs, while breed and sex of the host were not significantly associated with the occurrence of the disease. Mixed infection of B. gibsoni and B. vogeli was observed only in 3 dogs each in district Kasur and Rawalpindi. Our study is the first report to observe the occurrence of canine babesiosis in rural dogs in Pakistan through PCR.
犬巴贝斯虫病是一种重要的由蜱传播的犬类原生动物疾病，在全球范围内构成重大健康问题。农村地区的农场犬作为伴侣动物，不仅要看护畜群，还要守护主人的房屋。每个农民都饲养自己的伴侣犬以获得所有这些服务。在我们的研究中，从巴基斯坦旁遮普省三个不同生态区（该省南部、中部和北部地区）共采集了450份农场犬的血样，通过显微镜检查和聚合酶链反应（PCR）进行检测。薄血涂片检查显示犬巴贝斯虫寄生虫的总体患病率为12.8%（58/450）。然而，PCR分析显示，分别有46.8%（211/450）和7.3%（33/450）的样本对吉氏巴贝斯虫和韦氏巴贝斯虫呈阳性。通过PCR分别扩增出671 bp和590 bp的扩增子用于检测吉氏巴贝斯虫和韦氏巴贝斯虫。多变量分析结果表明，旁遮普中部地区犬巴贝斯虫病的发生率较高，且患病犬年龄较小，而宿主的品种和性别与该病的发生无显著关联。仅在卡苏尔区和拉瓦尔品第区各有3只犬观察到吉氏巴贝斯虫和韦氏巴贝斯虫的混合感染。我们的研究是首次通过PCR观察到巴基斯坦农村犬中犬巴贝斯虫病的发生情况的报告。
