Eamsobhana P, Yong H S, Song S L, Tungtrongchitr A, Roongruangchai K
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Trop Biomed. 2018 Sep 1;35(3):669-677.
Members of the genus Anisakis are parasitic nematodes of the family Anisakidae. They are potential zoonotic parasites, causing anisakiasis in humans who consume raw or undercooked seafood (fish or squid) infected with the larvae of this nematode. In the present study, anisakid nematodes collected from the marine fish Priacanthus tayenus (Purplespotted big-eye) caught from the Gulf of Thailand were examined morphologically and characterized genetically by DNA sequence analysis. Sequence data from the mitochondrial cytochrome c oxidase subunit II (mtDNA cox2) gene were used to identify these nematodes to species level and to evaluate the phylogenetic relationship among various taxa. All the 15 third-stage larvae of Anisakis nematodes investigated in this study belonged to the same genetic lineage as the A. typica species complex (named here as A. typica sp. T - T for Thailand). Eight mtDNA cox2 haplotypes were revealed in the 15 isolates of this Anisakis from Thailand. The mtDNA cox2 haplotypes of A. typica sp. T from Thailand were genetically distinct from those of the A. typica sensu stricto. Taxonomic description of this A. typica sp. T as a distinct species however awaits the availability of adult specimens.
异尖线虫属的成员是异尖科的寄生线虫。它们是潜在的人畜共患寄生虫，可在食用感染了这种线虫幼虫的生的或未煮熟的海鲜（鱼或鱿鱼）的人类中引起异尖线虫病。在本研究中，对从泰国湾捕获的海洋鱼类长尾大眼鲷（Priacanthus tayenus）中采集的异尖线虫进行了形态学检查，并通过DNA序列分析进行了基因特征鉴定。利用线粒体细胞色素c氧化酶亚基II（mtDNA cox2）基因的序列数据将这些线虫鉴定到种水平，并评估不同分类群之间的系统发育关系。本研究中调查的所有15条异尖线虫第三期幼虫与典型异尖线虫物种复合体属于同一遗传谱系（在此命名为典型异尖线虫泰国种A. typica sp. T）。在来自泰国的这15株异尖线虫分离物中发现了8种mtDNA cox2单倍型。泰国典型异尖线虫泰国种A. typica sp. T的mtDNA cox2单倍型在基因上与狭义的典型异尖线虫不同。然而，将这种典型异尖线虫泰国种A. typica sp. T描述为一个独特的物种还需等待成年标本的获得。
