Chaiphongpachara Tanawat, Adisakwattana Poom, Suwandittakul Nantana
Department of Public Health and Health Promotion, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Bangkok, Thailand.
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
ScientificWorldJournal. 2022 Jun 21;2022:2122619. doi: 10.1155/2022/2122619. eCollection 2022.
nematodes infecting Indian mackerel () were initially discovered in Thailand in our preliminary investigation. Nevertheless, the species of collected has not been determined nor has its genetic variation been researched. Thus, this study aimed to molecularly identify the species of specimens using the internal transcribed spacer (ITS) region of ribosomal DNA sequences. In addition, the intraspecific genetic variation was also determined using mitochondrial cytochrome oxidase subunit II (COII) gene sequences. The phylogenetic relationships of the ITS region classified all samples into ; however, the genetic variation between them could not be distinguished. By contrast, the phylogenetic tree analysis of the COII region identified all samples as . , with 17 different haplotypes by 66 polymorphic sites and five of the substitutions resulted in amino acid change. Additionally, the distribution pattern of the COII region can be separated into two groups between South America and Asian countries. All our haplotypes belong to Asian countries. Compared with the two genetic markers used in this investigation, COII appears to be a better candidate for studying genetic variation sensitive to environmental changes and intermediate or definitive host behavioral changes.
在我们的初步调查中，感染印度鲭（）的线虫最初是在泰国发现的。然而，所收集的 的物种尚未确定，其遗传变异也未得到研究。因此，本研究旨在利用核糖体DNA序列的内部转录间隔区（ITS）对 标本进行分子鉴定。此外，还利用线粒体细胞色素氧化酶亚基II（COII）基因序列确定种内遗传变异。ITS区域的系统发育关系将所有样本归类为 ；然而，它们之间的遗传变异无法区分。相比之下，COII区域的系统发育树分析将所有样本鉴定为 。 ，有17种不同的单倍型，由66个多态性位点组成，其中5个替换导致氨基酸变化。此外，COII区域的分布模式在南美洲和亚洲国家之间可分为两组。我们所有的单倍型都属于亚洲国家。与本研究中使用的两个遗传标记相比，COII似乎是研究对环境变化和中间宿主或终末宿主行为变化敏感的遗传变异的更好候选标记。
