M Beiromvand, Rafiei A, Mirzavand S, Rahdar M, Feiz Haddad M H
Infectious and Tropical Disease Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Trop Biomed. 2018 Mar 1;35(1):32-40.
Echinococcosis and toxocariasis are considered two important zoonotic diseases worldwide. The aim of the study was to evaluate the seroprevalence of cystic echinococcosis and toxocariasis and identify the role of variable factors such as age, gender, occupation, level of education, and having contact with domestic dogs and cats in the transmission of the parasites in rural inhabitants of Khuzestan Province, located in the southwest of Iran. A total of 410 rural inhabitants, who had contact with domestic dogs and cats, were randomly enrolled in the current study. Antigen B (AgB) was purified from the hydatid cyst fluid, and the ELISA test was performed for seropositive detection of cystic echinococcosis. Toxocara IgG was evaluated by ELISA DRG kit, and the positive samples were examined by Western blotting IgG LDBIO kit to confirm and validate the results of ELISA. Of the 410 sera examined, 20 (4.9%) samples were positive for cystic echinococcosis by ELISA test. A significant association was observed on the different type of occupations and seropositivity (p<0.05), but no significant differences were observed between age groups, gender, and educational levels for cystic echinococcosis. Eight (2.0%) cases were found to be positive for toxocariasis by ELISA, but none of the positive cases was confirmed by the Western blotting test. Our study showed that factors such as education, raising awareness, and health improvement in Khuzestan Province may have influenced the prevalence of echinococcosis and toxocariasis in recent years. Local health and sanitary authorities should pay more attention to the health problems in the rural areas.
棘球蚴病和弓蛔虫病被认为是全球两种重要的人畜共患病。本研究的目的是评估囊性棘球蚴病和弓蛔虫病的血清流行率，并确定年龄、性别、职业、教育水平以及与家犬和家猫接触等可变因素在伊朗西南部胡齐斯坦省农村居民寄生虫传播中的作用。共有410名与家犬和家猫有接触的农村居民被随机纳入本研究。从包虫囊肿液中纯化抗原B（AgB），并进行ELISA检测以血清学阳性检测囊性棘球蚴病。通过ELISA DRG试剂盒评估弓蛔虫IgG，并通过Western blotting IgG LDBIO试剂盒检测阳性样本以确认和验证ELISA结果。在检测的410份血清中，ELISA检测发现20份（4.9%）样本囊性棘球蚴病呈阳性。观察到不同职业类型与血清阳性之间存在显著关联（p<0.05），但在囊性棘球蚴病的年龄组、性别和教育水平之间未观察到显著差异。ELISA检测发现8例（2.0%）弓蛔虫病呈阳性，但Western blotting检测未确认任何阳性病例。我们的研究表明，胡齐斯坦省的教育、提高认识和改善健康等因素可能影响了近年来棘球蚴病和弓蛔虫病的流行率。当地卫生和卫生当局应更加关注农村地区的健康问题。
