马来西亚半岛啮齿动物中芽囊原虫的遗传多样性。
Genetic diversity of rodent Blastocystis sp. from Peninsular Malaysia.
作者信息
Farah Haziqah M T, Mohd Zain S N, Chandrawathani P, Premaalatha B, Mohd Khairul Nizam M K, Arutchelvan R, Suresh K
机构信息
Institute of Biological Sciences, Faculty of Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulalu Pinang, Malaysia.
出版信息
Trop Biomed. 2018 Jun 1;35(2):586-592.
Rodents are ubiquitous zoonotic vectors for many human pathogens including Blastocystis sp. In this study, we examined the prevalence and subtypes of Blastocystis sp. in rodents captured from Peninsular Malaysia. A total of 293 rodents predominantly brown rat (Rattus norvegicus) (290 of 293, 99.0%) and house shrew (Suncus murinus) (3 of 293, 1.0 %), were captured in the vicinity of popular eateries in two cities (Kuala Lumpur and Ipoh) in Peninsular Malaysia. In vitro cultivation method showed presence of Blastocystis sp. in approximately half (133 of 290, 45.9%) of the brown rats tested. Among the 47 Blastocystis isolates subtyped using partial small subunit ribosomal RNA gene analysis, ST4 was the most abundant (43 of 47, 91.5%) followed by ST1 (2 of 47, 4.3%), ST5 (1 of 47, 2.1%) and ST7 (1 of 47, 2.1%). Our findings highlighted the importance of rodents as a source of Blastocystis sp. infection in Malaysia and showed the high prevalence of ST4 within the rodent population infected with Blastocystis sp.
啮齿动物是包括芽囊原虫属在内的许多人类病原体普遍存在的人畜共患病媒介。在本研究中，我们调查了从马来西亚半岛捕获的啮齿动物中芽囊原虫属的流行情况和亚型。在马来西亚半岛两个城市（吉隆坡和怡保）的热门餐馆附近，共捕获了293只啮齿动物，主要是褐家鼠（293只中的290只，99.0%）和家鼩鼱（293只中的3只，1.0%）。体外培养方法显示，在大约一半（290只中的133只，45.9%）检测的褐家鼠中存在芽囊原虫属。在使用部分小亚基核糖体RNA基因分析进行亚型分类的47株芽囊原虫分离株中，ST4最为常见（47株中的43株，91.5%），其次是ST1（47株中的2株，4.3%）、ST5（47株中的1株，2.1%）和ST7（47株中的1株，2.1%）。我们的研究结果突出了啮齿动物作为马来西亚芽囊原虫属感染源的重要性，并显示了ST4在感染芽囊原虫属的啮齿动物群体中的高流行率。
Zotero 插件