Wang Jiayan, Wang Yiqing, Huang Wenwen, Zhang Ting, Yu Kuai, Chen Jiani, Zhou Liyuting, Cao Wenjie, Xu Junchen, Ma Jianshe, Huang Huicong, Zhao Wei
School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
Front Vet Sci. 2024 Jul 2;11:1427490. doi: 10.3389/fvets.2024.1427490. eCollection 2024.
Globally, rodents and shrew populations constitute crucial elements of diverse environments and animal communities. It is imperative to study their population dynamics to mitigate any potential negative impact on humans, as they can be involved in the transmission of critical zoonotic agents, such as . Therefore, this study aimed to identify the prevalence and genetic composition of in wild rodents and shrews residing in the Zhejiang provinces of China.
A total of 652 wild rodents and and shrews were captured from three different regions in Zhejiang Province from April 1st to October 31, 2023. The DNA was isolated by collecting fresh feces from the intestines of each rodent or and shrew. Rodent and shrew species were examined by vertebrate cytochrome b () analysis and PCR amplification. was also found in all fecal samples using PCR analysis and sequencing of the partial small subunit of ribosomal RNA () gene.
Among all the samples, 6.6% (43/652) showed a positive result for . In the results, 6 species of rodent and shrew were identified with , including ( = 36) (2.8%), ( = 75) (17.3%), ( = 18) (5.6%), ( = 155) (2.6%), ( = 86) (3.5%), and ( = 282) (7.4%). The existence of 6 subtypes, ST4 ( = 33), ST1 (4), ST7 ( = 3), ST2 ( = 1), ST3 ( = 1), and ST5 ( = 1), were confirmed by sequence analysis.
Based on the molecular data obtained, the wild rodents and shrews under investigation were found to be concurrently infected with zoonotic subtypes of , including ST1 to ST5 and ST7. This suggests that these animals could potentially pose a zoonotic threat to humans and other animals susceptible to infection.
在全球范围内，啮齿动物和鼩鼱种群是多样环境和动物群落的关键组成部分。研究它们的种群动态以减轻对人类的任何潜在负面影响至关重要，因为它们可能参与重要人畜共患病原体的传播，例如 。因此，本研究旨在确定中国浙江省野生啮齿动物和鼩鼱中 的流行率和基因组成。
2023年4月1日至10月31日，从浙江省三个不同地区捕获了总共652只野生啮齿动物和鼩鼱。通过从每只啮齿动物或鼩鼱的肠道收集新鲜粪便来分离DNA。通过脊椎动物细胞色素b（ ）分析和PCR扩增来检测啮齿动物和鼩鼱的物种。还使用PCR分析和核糖体RNA（ ）基因部分小亚基的测序在所有粪便样本中发现了 。
在所有样本中，6.6%（43/652）对 呈阳性结果。结果中，鉴定出6种携带 的啮齿动物和鼩鼱，包括 （ = 36）（2.8%）、 （ = 75）（17.3%）、 （ = 18）（5.6%）、 （ = 155）（2.6%）、 （ = 86）（3.5%）和 （ = 282）（7.4%）。通过序列分析确认存在6种 亚型，即ST4（ = 33）、ST1（4）、ST7（ = 3）、ST2（ = 1）、ST3（ = 1）和ST5（ = 1）。
根据获得的分子数据，发现所调查的野生啮齿动物和鼩鼱同时感染了 的人畜共患病亚型，包括ST1至ST5和ST7。这表明这些动物可能对人类和其他易感染 的动物构成人畜共患病威胁。
