仓鼠囊胚来源的胚胎干细胞的建立。

Establishment of hamster blastocyst-derived embryonic stem (ES) cells.

作者信息

Doetschman T, Williams P, Maeda N

机构信息

Medical Genetics Department, University of Wisconsin, Madison 53706.

出版信息

Dev Biol. 1988 May;127(1):224-7. doi: 10.1016/0012-1606(88)90204-7.

DOI:10.1016/0012-1606(88)90204-7

PMID:3360212

Abstract

The establishment of four ES cell lines from the Syrian "golden" hamster (Mesocricetus auratus) is described. The cells can be maintained in the undifferentiated state when grown on primary mouse embryonic fibroblast feeder layers. In suspension culture they spontaneously differentiate into embryoid bodies of increasing complexity which contain a variety of tissues including embryonic ectoderm and myocardium. All four lines--one female and three male--are karyotypically normal with 44 chromosomes. Hamster is the second species from which ES cells have been established. As in mouse, the cells should be useful for developmental and transgenic studies.

摘要

本文描述了从叙利亚“金色”仓鼠（Mesocricetus auratus）建立四个胚胎干细胞系的过程。这些细胞在原代小鼠胚胎成纤维细胞饲养层上生长时能够维持未分化状态。在悬浮培养中，它们会自发分化为复杂性不断增加的胚状体，其中包含多种组织，包括胚胎外胚层和心肌。所有四个细胞系——一个雌性和三个雄性——的染色体核型正常，有44条染色体。仓鼠是第二个建立胚胎干细胞系的物种。与小鼠一样，这些细胞应可用于发育研究和转基因研究。