Eckel R H, Goldberg I J, Steiner L, Yost T J, Paterniti J R
Department of Medicine, University of Colorado Health Sciences Center, Denver 80262.
Diabetes. 1988 May;37(5):610-5. doi: 10.2337/diab.37.5.610.
Lipolytic activity was measured in human plasma without prior administration of intravenous heparin. Eluted from heparin-Sepharose in a barbital buffer containing 6 mg/ml heparin, plasma lipolytic activities in 20 subjects were distributed between hepatic triglyceride lipase (HTGL, mean +/- SE 60.6 +/- 4.6%) and extrahepatic lipoprotein lipase (LPL, 39.4 +/- 4.6%). Confirmation of the identities of HTGL and LPL was provided by inhibitory antisera. Preheparin LPL activity was absent in plasma from a patient with type I hyperlipoproteinemia. Both preheparin HTGL and LPL activities correlated with the respective activities measured in plasma obtained 15 min after intravenous injection of heparin (rs = + .774 and + .685, respectively; n = 12). Evidence for the metabolic regulation of preheparin lipases was provided by measurement of significant increases in LPL and HTGL activities after oral glucose ingestion. Overall, preheparin plasma HTGL and LPL activities may reflect ongoing lipoprotein lipolytic activity in tissue beds, and because these measurements do not require the administration of intravenous heparin, they should prove useful for additional studies of short-term regulation of the lipases.
在未预先静脉注射肝素的情况下测量人血浆中的脂解活性。从含有6mg/ml肝素的巴比妥缓冲液中的肝素-琼脂糖凝胶上洗脱后，20名受试者的血浆脂解活性分布于肝甘油三酯脂肪酶（HTGL，平均值±标准误60.6±4.6%）和肝外脂蛋白脂肪酶（LPL，39.4±4.6%）之间。通过抑制性抗血清证实了HTGL和LPL的身份。一名I型高脂蛋白血症患者的血浆中不存在肝素前LPL活性。肝素前HTGL和LPL活性均与静脉注射肝素15分钟后获得的血浆中测量的各自活性相关（rs分别为+0.774和+0.685；n = 12）。口服葡萄糖后LPL和HTGL活性显著增加的测量结果为肝素前脂肪酶的代谢调节提供了证据。总体而言，肝素前血浆HTGL和LPL活性可能反映组织床中正在进行的脂蛋白脂解活性，并且由于这些测量不需要静脉注射肝素，它们应该对脂肪酶短期调节的进一步研究有用。
