College of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, USA.
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
Nat Commun. 2021 Feb 18;12(1):1123. doi: 10.1038/s41467-021-21449-0.
Bud-break is an economically and environmentally important process in trees and shrubs from boreal and temperate latitudes, but its molecular mechanisms are poorly understood. Here, we show that two previously reported transcription factors, EARLY BUD BREAK 1 (EBB1) and SHORT VEGETATIVE PHASE-Like (SVL) directly interact to control bud-break. EBB1 is a positive regulator of bud-break, whereas SVL is a negative regulator of bud-break. EBB1 directly and negatively regulates SVL expression. We further report the identification and characterization of the EBB3 gene. EBB3 is a temperature-responsive, epigenetically-regulated, positive regulator of bud-break that provides a direct link to activation of the cell cycle during bud-break. EBB3 is an AP2/ERF transcription factor that positively and directly regulates CYCLIND3.1 gene. Our results reveal the architecture of a putative regulatory module that links temperature-mediated control of bud-break with activation of cell cycle.
芽休眠是北方和温带树木和灌木中具有经济和环境意义的重要过程，但对其分子机制却知之甚少。在这里，我们表明，两个先前报道的转录因子，早期芽休眠 1（EBB1）和短营养期样（SVL）直接相互作用以控制芽休眠。EBB1 是芽休眠的正调节剂，而 SVL 是芽休眠的负调节剂。EBB1 直接和负调控 SVL 的表达。我们进一步报告了 EBB3 基因的鉴定和表征。EBB3 是一个对温度响应的、受表观遗传调控的芽休眠正调节剂，它提供了在芽休眠过程中激活细胞周期的直接联系。EBB3 是一个 AP2/ERF 转录因子，它正向和直接调控 CYCLIND3.1 基因。我们的结果揭示了一个假定的调控模块的结构，该模块将芽休眠的温度介导控制与细胞周期的激活联系起来。
