EGCG 调节在用于支架植入的 316L 不锈钢上生长的人脐静脉内皮细胞的细胞凋亡。
EGCG Regulates Cell Apoptosis of Human Umbilical Vein Endothelial Cells Grown on 316L Stainless Steel for Stent Implantation.
机构信息
Department of Cardiology, The Second Hospital of Jilin University, Changchun, 130041, People's Republic of China.
Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, 130021, People's Republic of China.
出版信息
Drug Des Devel Ther. 2021 Feb 11;15:493-499. doi: 10.2147/DDDT.S296548. eCollection 2021.
BACKGROUND
Nowadays, medical grade 316L stainless steel (316L SS) is being widely used for intravascular stents, and the drug-eluting stent (DES) system is able to significantly reduce the occurrences of in-stent restenosis. But the drugs and the polymer coating used in DES potentially induce the forming of late stent thrombosis. In order to reduce the occurrence of ISR after stent implantation, the development of novel drugs for DESs is urgently needed.
METHODS
This study aimed to investigate the potential mechanisms of epigallocatechin-3-gallate (EGCG) on human umbilical vein endothelial cells (HUVEC) grown on 316L stainless steel (316L SS) using flow cytometry and Q-PCR methods.
RESULTS
Our results showed that EGCG (12.5, 25, 50, 100 μmol/L) significantly inhibited HUVEC proliferation. Flow cytometry analysis indicated that EGCG (25, 50, 100 μmol/L) induced apoptosis. Moreover, qRT-PCRrevealed that genes associated with cell apoptosis (caspase-3, 8, 9, Fas) and autophagy (Atg 5, Atg 7, Atg 12) were up-regulated after EGCG treatment.
CONCLUSION
These findings indicate that EGCG possesses chemo preventive potential in stent coating which may serve as a novel new drug for stent implantation.
背景
如今，医用 316L 不锈钢（316L SS）被广泛用于血管内支架，药物洗脱支架（DES）系统能够显著降低支架内再狭窄的发生。但 DES 中使用的药物和聚合物涂层可能会导致晚期支架血栓形成。为了降低支架植入后的再狭窄发生率，迫切需要开发新型 DES 药物。
方法
本研究旨在使用流式细胞术和 Q-PCR 方法研究表没食子儿茶素没食子酸酯（EGCG）对生长在 316L 不锈钢（316L SS）上的人脐静脉内皮细胞（HUVEC）的潜在作用机制。
结果
我们的结果表明，EGCG（12.5、25、50、100 μmol/L）显著抑制 HUVEC 增殖。流式细胞术分析表明 EGCG（25、50、100 μmol/L）诱导细胞凋亡。此外，qRT-PCR 显示 EGCG 处理后与细胞凋亡（caspase-3、8、9、Fas）和自噬（Atg 5、Atg 7、Atg 12）相关的基因上调。
结论
这些发现表明 EGCG 在支架涂层中具有化学预防潜力，可能成为支架植入的新型新药。
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