Wang Xiaofeng, Hu Yan, Lu Xin, Cai Yu, Shu Jian
Department of Radiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
Exp Ther Med. 2021 Mar;21(3):225. doi: 10.3892/etm.2021.9656. Epub 2021 Jan 18.
The aim of the study was to explore the diagnostic value of T2 mapping in an experimental rat model of chronic liver disease. Chronic hepatitis was induced in Sprague-Dawley male rats (n=88) by intraperitoneal and abdominal subcutaneous injection of carbon tetrachloride in olive oil. The normal control rats (n=12) were similarly injected with the same dose of normal saline. All rats were randomly selected and subjected to T2-weighted/spectral adiabatic inversion recovery and multiple gradient- and spin-echo sequence. After scanning, rats were sacrificed immediately and livers removed for staining with hematoxylin and eosin, as well as Masson's trichrome, to determine the pathological stage of hepatic fibrosis, necroinflammatory activity and steatosis. The T2 values were measured and associated with histopathological findings. The T2 values were significantly associated with hepatic fibrosis (P<0.05), but not with hepatitis (P>0.05) or steatosis (P>0.05). By partial correlation analysis, a significant positive correlation was observed between the T2 values and stages of liver fibrosis (=0.820; P<0.05). T2 values increased with progressive hepatic fibrosis. The differences between T2 values and stages of liver fibrosis were statistically significant. Statistically significant differences were observed between different stages of liver fibrosis (P<0.05), with an area under the curve value of 0.944 for predicting stage F1 or greater, 0.942 for stage F2 or greater, 0.958 for stage F3 or greater, and 0.948 for F4. Thus, the T2 value is one of the quantitative indices of imaging and accurately reflects the stages of liver fibrosis.
本研究的目的是探讨T2映射在慢性肝病实验大鼠模型中的诊断价值。通过腹腔内和腹部皮下注射橄榄油中的四氯化碳，在Sprague-Dawley雄性大鼠（n = 88）中诱导慢性肝炎。正常对照大鼠（n = 12）同样注射相同剂量的生理盐水。所有大鼠均被随机选取并接受T2加权/频谱绝热反转恢复和多梯度及自旋回波序列检查。扫描后，立即处死大鼠并取出肝脏，用苏木精和伊红以及Masson三色染色，以确定肝纤维化的病理阶段、坏死性炎症活动和脂肪变性。测量T2值并将其与组织病理学结果相关联。T2值与肝纤维化显著相关（P<0.05），但与肝炎（P>0.05）或脂肪变性（P>0.05）无关。通过偏相关分析，观察到T2值与肝纤维化阶段之间存在显著正相关（=0.820；P<0.05）。T2值随肝纤维化进展而增加。T2值与肝纤维化阶段之间的差异具有统计学意义。在肝纤维化的不同阶段观察到统计学显著差异（P<0.05），预测F1或更高阶段的曲线下面积值为0.944，F2或更高阶段为0.942，F3或更高阶段为0.958，F4为0.948。因此，T2值是成像的定量指标之一，可准确反映肝纤维化的阶段。
