Ma Lulin, Li Juan, Zhou Junli, Zhang Dexin, Xiao Zhi, Yu Tian, Li Ying, Cao Song
Department of Pain Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
iScience. 2021 Jan 26;24(2):102108. doi: 10.1016/j.isci.2021.102108. eCollection 2021 Feb 19.
This study aimed to explore the effects and possible mechanisms of intravenous lidocaine in postherpetic neuralgia (PHN) rats. Mechanical withdrawal thresholds and thermal withdrawal latencies were measured. Open field test, elevated plus maze test, and tail suspension test were used to assess anxiety- and depressive-like behaviors. Microglia and astrocytes in spinal dorsal horn (SDH), prefrontal cortex (PFC), anterior cingulate cortex (ACC), and hippocampus were analyzed. The expression of TNF-α, IL-1β, and IL-4 in SDH and serum were evaluated. Intravenous lidocaine alleviated mechanical allodynia and thermal hypoalgesia, downregulated the expression of TNF-α and IL-1β, and inhibited the activation of microglia and astrocytes in SDH. In addition, it reduced the activation of astrocyte but not microglia in PFC, ACC, and hippocampus. Intravenous lidocaine may relieve PHN by inhibiting the activation of microglia and astrocyte in SDH or by reducing the neuroinflammation and astrocyte activation in PFC, ACC, and hippocampus.
本研究旨在探讨静脉注射利多卡因对带状疱疹后神经痛（PHN）大鼠的影响及可能机制。测量机械性撤针阈值和热缩足潜伏期。采用旷场试验、高架十字迷宫试验和悬尾试验评估焦虑样和抑郁样行为。分析脊髓背角（SDH）、前额叶皮质（PFC）、前扣带回皮质（ACC）和海马中的小胶质细胞和星形胶质细胞。评估SDH和血清中TNF-α、IL-1β和IL-4的表达。静脉注射利多卡因可减轻机械性异常性疼痛和热痛觉减退，下调TNF-α和IL-1β的表达，并抑制SDH中小胶质细胞和星形胶质细胞的激活。此外，它还减少了PFC、ACC和海马中星形胶质细胞的激活，但未减少小胶质细胞的激活。静脉注射利多卡因可能通过抑制SDH中小胶质细胞和星形胶质细胞的激活，或通过减少PFC、ACC和海马中的神经炎症和星形胶质细胞激活来缓解PHN。
