Histological Analysis of Gonads in Zebrafish.

Zebrafish is an excellent system for the study of gonad development due to available genetic tools and its utilization as a human disease model. The zebrafish serves as an experimental system to model human disorders affecting the reproductive system, toxicological effects on fertility and sexual development, and hormonal regulation of fertility. Forward genetic screens have been used to uncover genetic causes of infertility and reverse genetic approaches have demonstrated that genes involved in germ cell development have similar functions in zebrafish and mammals. The most comprehensive picture of the gonad can be visualized by histology. There are a variety of methods that give excellent histology of zebrafish gonads. Below are methods for two staining approaches for the histology of paraffin-embedded zebrafish gonads.