Microbiome Center, The Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel.
Methods Mol Biol. 2021;2243:123-141. doi: 10.1007/978-1-0716-1103-6_7.
In this chapter, we will present an outline of a typical experimental and bioinformatic workflow for identification of bacterial amplicon sequence variants (ASVs) present in a set of samples. This chapter is written from a bioinformatic point of view; therefore, the specific experimental protocols are not detailed, but rather the impact of various experimental decisions on the downstream analysis is described. Emphasis is made on the transition from reads to ASVs, describing the Deblur algorithm.
在本章中，我们将介绍一个典型的实验和生物信息学工作流程的概述，用于鉴定一组样本中存在的细菌扩增子序列变异体（ASV）。本章从生物信息学的角度编写；因此，没有详细描述具体的实验方案，而是描述了各种实验决策对下游分析的影响。重点放在从读取到 ASV 的转变上，并描述 Deblur 算法。
