Department of Biochemistry, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
Department of Hospital Pharmacy, Fukuoka University Hospital, Fukuoka, Japan.
PLoS One. 2021 Feb 19;16(2):e0246125. doi: 10.1371/journal.pone.0246125. eCollection 2021.
Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occurrence of DIA or to determine the drugs causing it, the development of diagnostic and predictive tools for allergic reactions is important. We demonstrated a novel method, termed high-sensitive allergy test (HiSAT), for the rapid diagnosis of allergy (within 1 hr; with true-positive diagnosis rates of 89% and 9% for patients with and without allergy-like symptoms, respectively). HiSAT analyzes the cell kinetics as an index against chemotactic factors in a patient's serum, as different from the diagnosis using conventional methods. Once allergy has occurred, HiSAT can be used to determine the causative medicine using culture supernatants incubated with the subject's lymphocytes and the test allergen. This test is more efficient (60%) than the lymphocyte transformation test (20%). Furthermore, in HiSAT, cell mobility significantly increases in a dose-dependent manner against supernatant incubated with lymphocytes from a subject with pollinosis collected at a time when the subject is without allergic symptoms and the antigen. The result demonstraed that HiSAT might be a promising method to rapidly diagnose DIA or to determine with high accuracy the antigen causing allergy.
药物诱导的过敏（DIA）是药物治疗中意外引发的一种严重的临床问题，需要解决，因为它会中断对原发疾病的治疗。由于常规过敏测试不足以准确预测 DIA 的发生或确定引起它的药物，因此开发用于过敏反应的诊断和预测工具非常重要。我们展示了一种称为高灵敏度过敏测试（HiSAT）的新方法，用于快速诊断过敏（在 1 小时内；对有和没有过敏样症状的患者的真阳性诊断率分别为 89%和 9%）。HiSAT 分析细胞动力学作为患者血清中趋化因子的指标，与使用传统方法的诊断不同。一旦发生过敏，HiSAT 可以使用培养上清液和测试过敏原与患者的淋巴细胞孵育来确定致病药物。与淋巴细胞转化试验（20%）相比，该试验的效率更高（60%）。此外，在 HiSAT 中，细胞迁移性对在患者没有过敏症状和抗原时收集的花粉症患者淋巴细胞孵育的上清液呈剂量依赖性显著增加。结果表明，HiSAT 可能是一种快速诊断 DIA 或准确确定引起过敏的抗原的有前途的方法。
