CRISPR 碱基编辑器筛选可大规模鉴定变异功能。
CRISPR base editor screens identify variant function at scale.
机构信息
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA.
出版信息
Mol Cell. 2021 Feb 18;81(4):647-648. doi: 10.1016/j.molcel.2021.01.036.
Abstract
Cuella-Martin et al. (2021) and Hanna et al. (2021) showcase CRISPR base editing in large-scale pooled screens in human cells to discover both loss- and gain-of-function variants, enabling protein structure/function insights and clinical variant interpretation.
摘要
Cuella-Martin 等人（2021 年）和 Hanna 等人（2021 年）展示了在人类细胞中的大规模 pooled 筛选中使用 CRISPR 碱基编辑技术来发现功能丧失和获得性功能变异体，从而能够深入了解蛋白质结构/功能，并进行临床变异体解释。
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