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2
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Perturbing proteomes at single residue resolution using base editing.使用碱基编辑技术在单个残基分辨率上扰乱蛋白质组。
Nat Commun. 2020 Apr 20;11(1):1871. doi: 10.1038/s41467-020-15796-7.
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Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells.利用 CRISPR 引导的脱氨酶在人类黑色素瘤细胞中生成的突变文库的单细胞分析。
Commun Biol. 2020 Apr 2;3(1):154. doi: 10.1038/s42003-020-0888-2.
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A CRISPR-based base-editing screen for the functional assessment of BRCA1 variants.基于 CRISPR 的 BRCA1 变体功能评估碱基编辑筛选。
Oncogene. 2020 Jan;39(1):30-35. doi: 10.1038/s41388-019-0968-2. Epub 2019 Aug 29.
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Accurate classification of BRCA1 variants with saturation genome editing.饱和基因组编辑精准分类 BRCA1 变异。
Nature. 2018 Oct;562(7726):217-222. doi: 10.1038/s41586-018-0461-z. Epub 2018 Sep 12.
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Multiplex assessment of protein variant abundance by massively parallel sequencing.通过大规模平行测序进行蛋白质变异体丰度的多重评估。
Nat Genet. 2018 Jun;50(6):874-882. doi: 10.1038/s41588-018-0122-z. Epub 2018 May 21.
8
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.基因组DNA中A•T到G•C的可编程碱基编辑，无需DNA切割。
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9
High-throughput Phenotyping of Lung Cancer Somatic Mutations.肺癌体细胞突变的高通量表型分析
Cancer Cell. 2016 Aug 8;30(2):214-228. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.在不进行双链DNA切割的情况下对基因组DNA中的目标碱基进行可编程编辑。
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CRISPR 碱基编辑器筛选可大规模鉴定变异功能。

CRISPR base editor screens identify variant function at scale.

机构信息

Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA.

Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA.

出版信息

Mol Cell. 2021 Feb 18;81(4):647-648. doi: 10.1016/j.molcel.2021.01.036.

DOI:10.1016/j.molcel.2021.01.036
PMID:33606973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9017793/
Abstract

Cuella-Martin et al. (2021) and Hanna et al. (2021) showcase CRISPR base editing in large-scale pooled screens in human cells to discover both loss- and gain-of-function variants, enabling protein structure/function insights and clinical variant interpretation.

摘要

Cuella-Martin 等人（2021 年）和 Hanna 等人（2021 年）展示了在人类细胞中的大规模 pooled 筛选中使用 CRISPR 碱基编辑技术来发现功能丧失和获得性功能变异体，从而能够深入了解蛋白质结构/功能，并进行临床变异体解释。