在实验室中进行 BARBEKO'ing：具有条形码碱基编辑器的多功能 CRISPR 筛选。
BARBEKO'ing in the lab: Versatile CRISPR screens with barcoded base editors.
机构信息
Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA; Department of Genetics, Harvard Medical School, Cambridge, MA, USA; Center for Bits and Atoms, Cambridge, MA, USA; MIT Media Lab, Massachusetts Institute of Technology, Cambridge, MA, USA.
出版信息
Mol Cell. 2021 Aug 5;81(15):3046-3047. doi: 10.1016/j.molcel.2021.07.007.
PMID:34358458
Abstract
Xu et al. (2021) describe a novel two-pronged CRISPR screen, termed BARBEKO, by coupling cytosine base editors and internally barcoded sgRNAs to eliminate double-stranded break-induced toxicity, enable high multiplicities of infection, and ensure experimental reproducibility.
摘要
徐等人（2021 年）描述了一种新型的双管齐下的 CRISPR 筛选方法，称为 BARBEKO，它通过将胞嘧啶碱基编辑器和内部 barcoded sgRNA 结合起来，消除双链断裂诱导的毒性，实现高感染复数，并确保实验的可重复性。
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