来自球形芽孢杆菌2362和2297的编码51.4千道尔顿和41.9千道尔顿蛋白质的杀蚊毒素基因的序列分析。
Sequence analysis of the mosquitocidal toxin genes encoding 51.4- and 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297.
作者信息
Baumann L, Broadwell A H, Baumann P
机构信息
Department of Bacteriology, University of California, Davis 95616.
出版信息
J Bacteriol. 1988 May;170(5):2045-50. doi: 10.1128/jb.170.5.2045-2050.1988.
Abstract
The nucleotide sequences of a 3,479-base-pair HindIII DNA fragment from Bacillus sphaericus 2362 and a 2,940-base-pair fragment from strain 2297 were determined; only minor differences were detected between them. Each contained two open reading frames coding for proteins of 51.4 and 41.9 kilodaltons. Both proteins were required for toxicity to larvae of the mosquito Culex pipiens.
摘要
测定了来自球形芽孢杆菌2362株的一个3479碱基对的HindIII DNA片段和来自2297株的一个2940碱基对片段的核苷酸序列；二者之间仅检测到微小差异。每个片段都包含两个编码51.4千道尔顿和41.9千道尔顿蛋白质的开放阅读框。这两种蛋白质对于致库蚊幼虫中毒都是必需的。
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