Chankamngoen Wasutorn, Janvilisri Tavan, Promdonkoy Boonhiang, Boonserm Panadda
Institute of Molecular Biosciences, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170 Thailand.
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400 Thailand.
3 Biotech. 2020 Aug;10(8):365. doi: 10.1007/s13205-020-02361-8. Epub 2020 Jul 31.
Binary or Bin toxin produced by is composed of BinA (42 kDa) and BinB (51 kDa) subunits. These work together to exert maximal toxicity against mosquito larvae via pore formation and induction of apoptosis. The C-terminal domains in both subunits are homologous to those of aerolysin-type β pore-forming toxins, including parasporin-2 (PS2). The latter is one of the toxins that exhibits specific cytotoxicity against human cancer cells. The present study investigates the possible anticancer activity of Bin toxin using PS2 as a control. We demonstrate that treatment with a high concentration of trypsin-activated Bin inhibits cell proliferation in human cancer cells A549, Caco-2, HepG2, HK-1 and KKU-M055. In the most susceptible cells, HK-1, Bin toxin exposure led to morphological alterations, decreased migration, decreased adhesion activity and apoptosis induction. Although these effects necessitated high concentrations, they suggest that Bin toxin may be optimized as a novel potential cancer-therapeutic agent.
由 产生的二元或Bin毒素由BinA（42 kDa）和BinB（51 kDa）亚基组成。它们共同作用，通过形成孔道和诱导细胞凋亡对蚊虫幼虫发挥最大毒性。两个亚基的C末端结构域与气单胞菌溶素型β孔形成毒素的结构域同源，包括副孢子素-2（PS2）。后者是对人类癌细胞具有特异性细胞毒性的毒素之一。本研究以PS2为对照，研究Bin毒素可能的抗癌活性。我们证明，用高浓度胰蛋白酶激活的Bin处理可抑制人癌细胞A549、Caco-2、HepG2、HK-1和KKU-M055中的细胞增殖。在最敏感的细胞HK-1中，暴露于Bin毒素会导致形态改变、迁移减少、粘附活性降低和细胞凋亡诱导。尽管这些作用需要高浓度，但它们表明Bin毒素可能被优化为一种新型潜在的癌症治疗剂。
