Shen Ya-Min, Gao Meng-Yue, Chen Xu, Shen Ai-Guo, Hu Ji-Ming
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; School of Printing and Packaging, Wuhan University, Wuhan 430079, PR China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 May 5;252:119566. doi: 10.1016/j.saa.2021.119566. Epub 2021 Feb 7.
Multiplex surface-enhanced Raman scattering (SERS) detection of markers without background in tumor biosystems has its superiority over other optical methods. Herein, we reported a strategy of quantitative discrimination of two breast cancer cell subtypes. Based on our previous studies, two kinds of Prussian blue analogue coated gold nanoparticles (Au@PBA NPs) were designed and synthesized by the replacement of Fe with Pb or Cu. Therefore, two distinct SERS emissions of C≡N bonds at 2122 cm and 2176 cm have been acquired. When modified with aptamers of epithelial cell adhesion molecule (EpCAM) and epidermal growth factor receptor (EGFR), which are both expressed in MCF-7 and MDA-MB-231 cell lines but in different levels, the SERS nanoprobes simultaneously identified the relative expression of these biomarkers on the cell surface, providing a good example for ratiometric detection in biosystems without any interference. Each surface marker of tumor cells corresponds to a single SERS emission. Thus, each subtype could be described in a molecular profiling way through duplex C≡N bonds-based SERS emission, which is more advanced than traditional flow cytometry method.
在肿瘤生物系统中，多重表面增强拉曼散射（SERS）对标志物进行无背景检测比其他光学方法具有优势。在此，我们报道了一种定量区分两种乳腺癌细胞亚型的策略。基于我们之前的研究，通过用Pb或Cu替代Fe，设计并合成了两种普鲁士蓝类似物包覆的金纳米颗粒（Au@PBA NPs）。因此，获得了在2122 cm和2176 cm处C≡N键的两种不同的SERS发射。用上皮细胞粘附分子（EpCAM）和表皮生长因子受体（EGFR）的适配体进行修饰后，这两种适配体在MCF-7和MDA-MB-231细胞系中均有表达但水平不同，SERS纳米探针同时识别了这些生物标志物在细胞表面的相对表达，为无任何干扰的生物系统中的比率检测提供了一个很好的例子。肿瘤细胞的每个表面标志物对应于单一的SERS发射。因此，每种亚型都可以通过基于双链C≡N键的SERS发射以分子谱的方式进行描述，这比传统的流式细胞术方法更先进。
