Lucio Marianna, Fick Alexander, Michalke Bernhard, Laemmer Robert, Hosari Sami, Hohberger Bettina
Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Department of Ophthalmology, University of Erlangen-Nürnberg, Erlangen, Germany.
Klin Monbl Augenheilkd. 2022 Mar;239(3):326-330. doi: 10.1055/a-1333-2816. Epub 2021 Feb 19.
Trace elements are assumed to be involved in glaucoma pathogenesis via changes in oxidative stress. Especially serum selenium (Se) has been linked to this neurodegenerative disease. Serum Se levels differ between countries due to nutrition and ethnicity. It was the aim of the present study to investigate serum Se levels in primary open-angle glaucoma (POAG) patients and controls in Germany and to consider potential age and gender effects.
The Se concentration of 39 serum samples (22 patients with POAG, 17 controls) were analyzed by inductively coupled plasma-sector field mass spectrometry (ICP-sf-MS) in high resolution mode. Covariance and percentile regression were analyzed. Age and gender were defined as confounding factors and their different trends were investigated. Moreover, age was examined across different quantiles of Se levels.
Total serum least-squares means (LS-means) Se levels were 132.02 µg/L (controls) and 134.86 µg/L (POAG). Total serum Se levels did not differ between the study groups (p > 0.05). Significant age and gender effects of serum Se were observed. Quantile analysis showed that the 1st serum Se quantile decreased with increasing age in POAG patients in contrast to controls. The odds ratios of the 1st serum Se were 1.3 (with 2nd quantile) and 1.3 (with 3rd quantile), respectively.
The serum Se level of the German cohort was almost half of those of the published US cohort (glaucoma 209.11 ng/mL; control 194.45 ng/mL). Age and gender effects were observed; the serum Se level increased with age in women (controls and POAG), however, Se levels decreased with age in men (controls and POAG).
微量元素被认为通过氧化应激的变化参与青光眼的发病机制。特别是血清硒（Se）已与这种神经退行性疾病相关联。由于营养和种族差异，不同国家的血清硒水平有所不同。本研究的目的是调查德国原发性开角型青光眼（POAG）患者和对照组的血清硒水平，并考虑潜在的年龄和性别影响。
采用高分辨率模式的电感耦合等离子体质谱仪（ICP-sf-MS）分析39份血清样本（22例POAG患者，17例对照）的硒浓度。分析协方差和百分位数回归。将年龄和性别定义为混杂因素，并研究它们的不同趋势。此外，还对不同硒水平分位数的年龄进行了检查。
血清总最小二乘均值（LS均值）硒水平在对照组为132.02μg/L，在POAG组为134.86μg/L。研究组之间的血清总硒水平无差异（p>0.05）。观察到血清硒存在显著的年龄和性别影响。分位数分析表明，与对照组相比，POAG患者中血清硒的第一个分位数随年龄增加而降低。血清硒第一个分位数的优势比分别为1.3（与第二个分位数相比）和1.3（与第三个分位数相比）。
德国队列的血清硒水平几乎是已发表的美国队列（青光眼209.11ng/mL；对照194.45ng/mL）的一半。观察到年龄和性别影响；女性（对照组和POAG组）的血清硒水平随年龄增加而升高，然而，男性（对照组和POAG组）的硒水平随年龄降低。
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