Department of Ophthalmology, University of Erlangen, Schwabachanlage 6, 91054 Erlangen, Germany.
University of Rostock, Doberaner Straße 140, 18057 Rostock, Germany.
J Trace Elem Med Biol. 2019 May;53:129-134. doi: 10.1016/j.jtemb.2019.02.006. Epub 2019 Feb 15.
Glaucoma disease is known as multifactorial. Trace elements seemed to be linked via oxidative stress mediated changes to the complex glaucoma pathophysiology. Thus, it was the aim of this study to investigate serum levels of trace elements in primary open-angle glaucoma patients (POAG).
Peripheral venous blood samples were taken from a total of 40 subjects: 22 primary open-angle glaucoma patients (mean age 58.1 ± 13.9, female 8, male 14) and 18 controls (mean age 38.9 ± 11.6, 6 female 6, male 12). Serum samples of cadmium, cobalt, copper, iron, lead, manganese and zinc were analyzed by Inductively-Coupled-Plasma-Optical Emission Spectrometry (Cu, Fe, Zn) and Inductively-Coupled-Plasma-sectorfield-Mass-Spectrometry (Cd, Co, Mn, Pb, Se). Data were analyzed using ANCOVA and presented as log transformed LS-mean.
Patients with POAG had significantly increased serum levels of iron (2.98 ± 0.03 μg/L vs 2.98 ± 0.03 μg/L) when compared to controls, and of cadmium (1.57 ± 0.05 ng/L vs. 1.40 ± 0.06 ng/L) considering the interaction between age and the class variable (control versus POAG). A gender effect was seen for cadmium, cobalt, copper, and iron in controls and POAG patients. Iron concentration was reduced in dependency of age for both genders in normals, however lesser in POAG patients. No difference was seen in serum levels of lead, manganese, and zinc between patients with POAG and controls.
A significant elevation of serum cadmium and iron levels in POAG patients as well as an additional gender effect of cadmium, cobalt, copper, and iron in normals and POAG patients, may argue for a potential role of these trace elements in the pathogenesis of primary open-angle glaucoma.
青光眼被认为是一种多因素疾病。微量元素似乎通过氧化应激介导的变化与复杂的青光眼病理生理学有关。因此，本研究旨在探讨原发性开角型青光眼（POAG）患者血清微量元素水平。
共采集 40 例患者的外周静脉血样本：22 例原发性开角型青光眼患者（平均年龄 58.1±13.9，女性 8 例，男性 14 例）和 18 例对照（平均年龄 38.9±11.6，女性 6 例，男性 12 例）。采用电感耦合等离子体发射光谱法（Cu、Fe、Zn）和电感耦合等离子体质谱法（Cd、Co、Mn、Pb、Se）分析血清样本中的镉、钴、铜、铁、铅、锰和锌。采用协方差分析（ANCOVA）对数据进行分析，并以对数转换后的最小二乘均数（LS-mean）表示。
与对照组相比，POAG 患者的血清铁水平明显升高（2.98±0.03μg/L 比 2.98±0.03μg/L），并且考虑到年龄和类别变量（对照组与 POAG）之间的相互作用，血清镉水平也升高（1.57±0.05ng/L 比 1.40±0.06ng/L）。对照组和 POAG 患者中，镉、钴、铜和铁存在性别效应。在正常人群中，铁浓度随年龄的增加而降低，但 POAG 患者降低幅度较小。POAG 患者和对照组之间的血清铅、锰和锌水平无差异。
POAG 患者血清镉和铁水平显著升高，以及正常人和 POAG 患者中镉、钴、铜和铁的额外性别效应，可能表明这些微量元素在原发性开角型青光眼的发病机制中起潜在作用。
