Department of Chemistry, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
J Biomed Mater Res B Appl Biomater. 2021 Oct;109(10):1578-1587. doi: 10.1002/jbm.b.34816. Epub 2021 Feb 19.
Targeted drug delivery enhances drug efficiency and selectivity without affecting normal cells. Luminescent nanoparticles can be used for tumor imaging as well as selective tumor targeting for drug delivery. In this research, LaVO :Eu was synthesized, the luminescent nanocrystal was coated by surface polymerization of levodopa in the presence of Paclitaxel (PTX), and then NL2 peptide was coupled on the surface of polymer-coated luminescent nanoparticles. Next, the capability of the modified drug was examined by in vitro and in vivo experiments. MTT assay on SK-BR-3 cell line (as breast cancer cells) and fluorescent microscopy results indicate that this modification decreases significantly drug toxicity and increases its selectivity. In addition, in vivo experiments confirm more capability of the NL2-functionalized nanocomposite for reducing tumor size, drug distribution in the body, and more aggregation of PTX in tumor tissue. Overall, it is concluded that tumor imaging is possible using luminescent LaVO :Eu core and NL2 peptide increases significantly the specificity of PTX in combination with a functionalized luminescent polymeric carrier.
靶向药物输送提高了药物的效率和选择性，而不会影响正常细胞。发光纳米粒子可用于肿瘤成像以及药物输送的选择性肿瘤靶向。在这项研究中，合成了 LaVO:Eu，在左旋多巴的表面聚合存在下，将发光纳米晶包覆，然后将 NL2 肽偶联到聚合物包覆的发光纳米粒子的表面。接下来，通过体外和体内实验来检查修饰药物的能力。MTT 法测定 SK-BR-3 细胞系（作为乳腺癌细胞）和荧光显微镜结果表明，这种修饰显著降低了药物毒性并提高了其选择性。此外，体内实验证实了 NL2 功能化纳米复合材料在减少肿瘤大小、药物在体内分布以及 PTX 在肿瘤组织中聚集方面的更大能力。总的来说，结论是使用发光 LaVO:Eu 核进行肿瘤成像，以及结合功能化发光聚合物载体，NL2 肽显著提高了 PTX 的特异性。
