Department of Urology, The Second Affiliated Hospital of Nanchang University, 1 Mingde RD, Nanchang, 310003, China.
School of Inter-Cultural Studies, Jiangxi Normal University, Nanchang, 330022, China.
Arch Pharm Res. 2021 Feb;44(2):219-229. doi: 10.1007/s12272-021-01310-0. Epub 2021 Feb 20.
MicroRNA(miR)-340 is known as a multifunctional miRNA related to various types of cancer while its role in renal cell carcinoma (RCC) remains to be further investigated. In the present study, an apparent increase in miR-340 expression was observed in both clear cell RCC tissues and RCC cell line 786-O and Caki-1. Functionally, the overexpression of miR-340 promoted cell proliferation, migration, invasion, extracellular alanine (Ala) level, and glycolysis level in 786-O cells. Then, frizzled class receptor 3 (FZD3) was determined as the target gene of miR-340 and its expression level was negatively regulated by miR-340. The FZD3 silencing abrogated the inhibitory effect of miR-340 knockdown on cell proliferation, migration, invasion, Ala level, and glycolysis level in 786-O cells. In conclusion, miR-340 promotes proliferation, migration, and invasion of RCC cells via suppressing FZD3 expression, and the promotion effect of miR-340 on RCC progression may be due to its regulatory effect on glycolysis and Ala level.
微小 RNA（miR）-340 是一种多功能 miRNA，与多种类型的癌症有关，但其在肾细胞癌（RCC）中的作用仍需进一步研究。在本研究中，在透明细胞 RCC 组织和 RCC 细胞系 786-O 和 Caki-1 中均观察到 miR-340 表达明显增加。功能上，miR-340 的过表达促进了 786-O 细胞的增殖、迁移、侵袭、细胞外丙氨酸（Ala）水平和糖酵解水平。然后，确定卷曲蛋白受体 3（FZD3）为 miR-340 的靶基因，其表达水平受 miR-340 负调控。FZD3 沉默消除了 miR-340 敲低对 786-O 细胞增殖、迁移、侵袭、Ala 水平和糖酵解水平的抑制作用。总之，miR-340 通过抑制 FZD3 表达促进 RCC 细胞的增殖、迁移和侵袭，miR-340 对 RCC 进展的促进作用可能归因于其对糖酵解和 Ala 水平的调节作用。
