大肠杆菌喹啉酸合成中天冬氨酸与磷酸二羟丙酮的缩合模式。
The mode of condensation of aspartic acid and dihydroxyacetone phosphate in quinolinate synthesis in Escherichia coli.
作者信息
Wicks F D, Sakakibara S, Gholson R K, Scott T A
出版信息
Biochim Biophys Acta. 1977 Nov 7;500(1):213-6. doi: 10.1016/0304-4165(77)90061-7.
PMID:336100
Abstract
Dihydroxy [3-14C]acetone phosphate was prepared enzymatically from [1-14C]glucose and use as a substrate in a partially purified quinolinate synthetase system prepared from Escherichia coli mutants. Carbon-by-carbon degradation of the resulting [14C]quinolinate showed that 96% of the 14C was located in carbon-4, indicating that carbon-3 of dihydroxyacetone phosphate condenses with carbon-3 of aspartate in quinolinate synthesis in E. coli.
摘要
磷酸二羟基[3-¹⁴C]丙酮由[1-¹⁴C]葡萄糖经酶促反应制备而成，并用作从大肠杆菌突变体中制备的部分纯化喹啉酸合成酶系统的底物。对所得[¹⁴C]喹啉酸进行逐碳降解分析，结果表明，96%的¹⁴C位于碳-4位，这表明在大肠杆菌喹啉酸合成过程中，磷酸二羟基丙酮的碳-3位与天冬氨酸的碳-3位发生了缩合反应。
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