Marine College, Shandong University, Weihai, Shandong, 264209, PR China.
Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Street, Harbin 150030, PR China.
Ecotoxicol Environ Saf. 2021 Apr 15;213:112048. doi: 10.1016/j.ecoenv.2021.112048. Epub 2021 Feb 19.
We conducted a large-scale epidemiological investigation to detect the prevalence of Toxoplasma gondii in four marine bivalve shellfish species collected from six representative coastal regions of Weihai, eastern China. Between January 2018 and December 2018, 14,535 marine bivalve shellfish pooled into 2907 samples were randomly collected and examined for T. gondii DNA by a nested PCR assay targeting B1 gene. The results showed that 2.8% (82) of the 2907 pooled samples were tested positive for T. gondii DNA. Two T. gondii genotype (ToxoDB Genotype #9 and ToxoDB Genotype #1) were identified PCR-restriction fragment length polymorphism analysis. Factors that were found significantly associated with the presence of T. gondii DNA in marine bivalve shellfish included the source of samples (being wild) (odds ratio [OR], 3.34; 95% confidence interval [CI], 2.00-5.84; p < 0.01), surface runoff near the sampling site (OR, 2.64; 95% CI, 1.47-4.72; p < 0.01), and presence of cats near the sampling site (OR, 1.77; 95% CI, 1.02-3.07; p = 0.04). Moreover, the prevalence of T. gondii DNA in marine bivalve shellfish correlated with temperature (Pearson's correlation: R = 0.75, p = 0.0049) and precipitation (R = 0.87, p = 0.00021). These findings provide new insights into the presence of T. gondii DNA in marine bivalve shellfish and highlight the impact of human activity on marine pollution by such an important terrestrial pathogen pollutant.
我们进行了一项大规模的流行病学调查，以检测来自中国东部威海六个代表性沿海地区的四种海洋双壳贝类中弓形虫的流行率。在 2018 年 1 月至 2018 年 12 月期间，我们随机采集了 14535 个海洋双壳贝类，汇集到 2907 个样本中，通过针对 B1 基因的巢式 PCR 检测来检测 T. gondii DNA。结果显示，2907 个汇集样本中有 2.8%（82 个）检测出 T. gondii DNA 阳性。通过 PCR-限制性片段长度多态性分析鉴定了两种 T. gondii 基因型（ToxoDB 基因型#9 和 ToxoDB 基因型#1）。通过多因素分析发现，与海洋双壳贝类中存在 T. gondii DNA 显著相关的因素包括样本来源（野生）（比值比 [OR]，3.34；95%置信区间 [CI]，2.00-5.84；p<0.01）、采样点附近的地表径流（OR，2.64；95% CI，1.47-4.72；p<0.01）和采样点附近有猫（OR，1.77；95% CI，1.02-3.07；p=0.04）。此外，海洋双壳贝类中 T. gondii DNA 的流行率与温度（Pearson 相关：R=0.75，p=0.0049）和降水（R=0.87，p=0.00021）相关。这些发现为海洋双壳贝类中 T. gondii DNA 的存在提供了新的见解，并强调了这种重要的陆地病原体污染物对海洋污染的人类活动的影响。
