中国东部江苏省病猪和流浪猫中弓形虫的流行情况、风险因素及遗传特征。

Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, eastern China.

机构信息

College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu Province 225000, PR China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu Province, 225000, PR China.

出版信息

Infect Genet Evol. 2018 Jun;60:17-25. doi: 10.1016/j.meegid.2018.02.007. Epub 2018 Feb 13.

DOI:10.1016/j.meegid.2018.02.007

PMID:29452292

Abstract

Toxoplasma gondii is an obligate intracellular parasitic protozoan with a worldwide distribution. The parasites in edible tissues of pigs and oocysts from cats are the major sources of T. gondii infection in humans. However, there are no data from sick pigs in veterinary clinics or from stray cats in Jiangsu Province, eastern China. In total, biological samples from 141 sick pigs and 64 stray cats were collected from this region. The rate of T. gondii infection in sick pigs was 46.81% using a polymerase chain reaction (PCR), and the overall prevalence of toxoplasmosis in stray cats was 34.38% by PCR and an enzyme-linked immunosorbent assay (ELISA). T. gondii was significantly more prevalent in lungs and heart than in liver and spleen (P < 0.05). Age and geographic region were considered to be the main risk factors associated with T. gondii infection in these pigs. The DNA samples from 17 sick pigs and seven stray cats, were successfully genotyped by multilocus PCR-restriction fragment length polymorphism (PCR-RFLP) with 10 genetic markers [SAG1, SAG2 (5'-3'SAG2, alt. SAG2), SAG3, GRA6, PK1, c22-8, c29-2, BTUB, L358 and Apico]. Six distinct genotypes were found, which were designated ToxoDB PCR-RFLP genotypes #9 (Chinese I), #10 (Type I), #213, and #89, and New 1 and New 2. Chinese I is the most prevalent T. gondii genotype in this region. The two new genotypes (designated New 1 and New 2) are reported and the ToxoDB PCR-RFLP genotype #89 is found for the first time in China. Such information will be useful for the prevention, diagnosis and treatment of porcine toxoplasmosis in Jiangsu Province, eastern China.

摘要

刚地弓形虫是一种全球性分布的专性细胞内寄生的原生动物。猪可食组织中的寄生虫和猫粪便中的卵囊是人类感染刚地弓形虫的主要来源。然而，目前江苏省兽医诊所的病猪和流浪猫中还没有相关数据。共采集了该地区 141 头病猪和 64 只流浪猫的生物样本。通过聚合酶链反应（PCR）检测，病猪的刚地弓形虫感染率为 46.81%，通过 PCR 和酶联免疫吸附试验（ELISA）检测，流浪猫的弓形虫总感染率为 34.38%。在肺和心脏中的感染率显著高于肝脏和脾脏（P < 0.05）。年龄和地理位置被认为是这些猪感染刚地弓形虫的主要危险因素。通过使用 10 个遗传标记（SAG1、SAG2（5'-3'SAG2，alt. SAG2）、SAG3、GRA6、PK1、c22-8、c29-2、BTUB、L358 和 Apico）的多位点 PCR-限制性片段长度多态性（PCR-RFLP），对 17 头病猪和 7 只流浪猫的 DNA 样本进行了成功的基因分型。发现了 6 种不同的基因型，分别命名为 ToxoDB PCR-RFLP 基因型 #9（中国 I）、#10（I 型）、#213、#89 和新 1 型和新 2 型。中国 I 是该地区最流行的刚地弓形虫基因型。新发现的两种基因型（命名为新 1 型和新 2 型），以及在中国首次发现的 ToxoDB PCR-RFLP 基因型 #89。这些信息将有助于预防、诊断和治疗江苏省的猪弓形虫病。