利用干血斑进行 SARS-CoV-2 抗体的血清学评估。
The use of dried blood spots for the serological evaluation of SARS-CoV-2 antibodies.
机构信息
Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.
Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.
出版信息
J Public Health (Oxf). 2022 Jun 27;44(2):e260-e263. doi: 10.1093/pubmed/fdab011.
BACKGROUND
To determine if dried blood spot specimens (DBS) can reliably detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies, we compared the SARS-CoV-2 IgG antibody response in paired serum and eluates from DBS specimens.
METHODS
A total of 95 paired DBS and serum samples were collected from 74 participants (aged 1-63 years) as part of a household cohort study in Melbourne, Australia. SARS-CoV-2 IgG antibodies specific for the receptor-binding domain (RBD) and S1 proteins between serum and eluates from DBS specimens were compared using an FDA-approved ELISA method.
RESULTS
Among the 74 participants, 42% (31/74) were children and the rest were adults. A total of 16 children and 13 adults were SARS-CoV-2 positive by polymerase chain reaction. The IgG seropositivity rate was similar between serum and DBS specimens (18.9% (18/95) versus 16.8% (16/95)), respectively. Similar RBD and S1-specific IgG levels were detected between serum and DBS specimens. Serum IgG levels strongly correlated with DBS IgG levels (r = 0.99, P < 0.0001) for both SARS-CoV-2 proteins. Furthermore, antibodies remained stable in DBS specimens for >3 months.
CONCLUSIONS
DBS specimens can be reliably used as an alternative to serum samples for SARS-CoV-2 antibody measurement. The use of DBS specimens would facilitate serosurveillance efforts particularly in hard-to-reach populations and inform public health responses including COVID-19 vaccination strategies.
背景
为了确定干血斑标本（DBS）是否能可靠地检测到严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）抗体，我们比较了血清和 DBS 标本洗脱液中 SARS-CoV-2 IgG 抗体的反应。
方法
本研究共纳入了来自澳大利亚墨尔本家庭队列研究中的 74 名参与者（年龄 1-63 岁）的 95 对 DBS 和血清样本。使用美国食品药品监督管理局（FDA）批准的 ELISA 方法比较了血清和 DBS 标本洗脱液中针对受体结合域（RBD）和 S1 蛋白的 SARS-CoV-2 IgG 抗体。
结果
在 74 名参与者中，42%（31/74）为儿童，其余为成年人。共有 16 名儿童和 13 名成年人经聚合酶链反应（PCR）检测为 SARS-CoV-2 阳性。血清和 DBS 标本的 IgG 血清阳性率分别为 18.9%（18/95）和 16.8%（16/95），差异无统计学意义。血清和 DBS 标本中均检测到相似的 RBD 和 S1 特异性 IgG 水平。血清 IgG 水平与 DBS IgG 水平强烈相关（两种蛋白 r 值分别为 0.99，P<0.0001）。此外，抗体在 DBS 标本中可稳定存在>3 个月。
结论
DBS 标本可作为 SARS-CoV-2 抗体检测的替代血清样本。DBS 标本的使用将特别有利于难以接触到的人群的血清学监测，并为包括 COVID-19 疫苗接种策略在内的公共卫生应对措施提供信息。
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