Miyoshi N, Matsumoto N, Hisazumi H, Fukuda M
Department of Pathology, Fukui Medical School, Japan.
Int J Hyperthermia. 1988 Mar-Apr;4(2):203-9. doi: 10.3109/02656738809029310.
Mouse leukaemia L1210 cells were subjected to hyperthermia (43.0 +/- 0.05 degrees C, water bath) and photodynamic therapy (50 micrograms/ml haematoporphyrin derivative (HpD), 630 +/- 5 nm at 0.1 mW/cm2) for varying lengths of treatment time in different sequences. Dose-response curves showed that the two modalities interact to make the combination more cytotoxic than the sum of the separate individual treatments, and that a determining factor is the intracellular concentration of HpD of tumour cells during hyperthermia. High cytotoxic (synergistic) effects of photodynamic therapy (PDT) were obtained when PDT was performed after hyperthermia following HpD administration. However, only additive cytotoxic effects were found, when hyperthermia was performed after HpD administration and PDT. These cytotoxic data were supported by DNA damage (the increase in single-stranded DNA) as revealed by flow cytometry in cells stained with acridine orange (AO). These in vitro experiments suggest that clinical applications of PDT after hyperthermia with HpD injection might be useful.
将小鼠白血病L1210细胞置于高温环境（43.0±0.05摄氏度，水浴）和光动力疗法（50微克/毫升血卟啉衍生物（HpD），630±5纳米，0.1毫瓦/平方厘米）下，以不同顺序进行不同时长的治疗。剂量反应曲线表明，这两种治疗方式相互作用，使联合治疗比单独的个体治疗更具细胞毒性，并且一个决定性因素是高温期间肿瘤细胞内HpD的浓度。在给予HpD后进行高温治疗后再进行光动力疗法（PDT）时，可获得较高的细胞毒性（协同）效应。然而，当在给予HpD和PDT后进行高温治疗时，仅发现相加的细胞毒性效应。这些细胞毒性数据得到了吖啶橙（AO）染色细胞的流式细胞术所揭示的DNA损伤（单链DNA增加）的支持。这些体外实验表明，注射HpD后进行高温治疗后再进行PDT的临床应用可能是有用的。
