Morimoto Y
Department of Obstetrics and Gynecology, Kansai Medical University, Osaka.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Feb;40(2):201-8.
Explants of human endometrium and decidua were cultured for 96 hours using the organ culture method. The tissues were observed under light and electron microscopes. The prolactin concentrations of the mediums and of the tissues were assayed with I131-prolactin kits. The prolactin concentrations in the mediums continued to increase during the 96-hour organ culture to 16ng/100mg w.w. for the proliferative endometrium, 200ng/100mg w.w. for the late secretory endometrium and 5.5 micrograms/100mg w.w. for the early gestational decidua. Ultrastructurally, each endometrial stromal cell and decidual stromal cell revealed its secretory appearance. For instance, after the culture there was an increase in ribosomes and Golgi apparatus, and the cisternae of the rough endoplasmic reticulum (r-ER) expanded. The differentiation of the stromal cells into fully developed decidual cells in ultrastructure was proportional to the increase in prolactin concentrations in the medium during the culture.
采用器官培养法将人子宫内膜和蜕膜外植体培养96小时。在光学显微镜和电子显微镜下观察组织。用I131-催乳素试剂盒检测培养基和组织中的催乳素浓度。在96小时的器官培养过程中，培养基中的催乳素浓度持续升高，增殖期子宫内膜达到16ng/100mg湿重，分泌晚期子宫内膜达到200ng/100mg湿重，妊娠早期蜕膜达到5.5微克/100mg湿重。在超微结构上，每个子宫内膜基质细胞和蜕膜基质细胞都呈现出分泌状态。例如，培养后核糖体和高尔基体增加，粗面内质网池扩张。培养过程中，基质细胞在超微结构上分化为完全成熟的蜕膜细胞的程度与培养基中催乳素浓度的增加成正比。
