Zaib Maryam, Sharif Ali, Akhtar Bushra, Khan Humaira Majeed, Akhtar Muhammad Furqan, Hassan Waseem, Razzaq Faisal, Nawaz Sajid, Qaisar Naeem
Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan.
Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Lahore.
Pak J Pharm Sci. 2020 Jul;33(4(Supplementary)):1805-1813.
Berberis lycium Royle (Berberidaceae) is traditionally used for the treatment of diabetes mellitus. Present study was conducted to determine the antioxidant, antidiabetic and anti-inflammatory effects of aqueous and methanolic whole plant extracts. Total phenolic contents were determined by Folin-ciocalteu method whereas antioxidant activity was determined by 2,2-diphenyl-1-picryl hydrazyl (DPPH) method. In vitro anti-diabetic activity was determined using alpha amylase assay. Acute hypoglycemic activity was investigated on normoglycemic rats. Sub-acute anti-diabetic effects were investigated in alloxan induced diabetic rats for 14 days. Methanolic extract exhibited 183.5±1 mg/g Gallic acid equivalent (GAE) phenolic contents. The methanolic extract exhibited an IC50 of 242µg/mL and 37.26 mg/mL in antioxidant and alpha amylase inhibitory assays respectively. Administration of methanolic extract in normoglycemic rats exhibited significant anti-hyperglycemic effect at 90 and 120 min. Methanolic extract (500 mg/kg extract) significantly reduced blood glucose at day 14. Methanolic extract (500 mg/kg) significantly reduced the concentration of tumor necrosis factor (TNF-α) and interleukin (IL-6) along with reduction in total cholesterol and triglyceride levels in diabetic rats. Administration of methanol extract also improved the hepatic markers. The study suggested that the methanolic extract possessed antidiabetic effect that might be attributed to its alpha amylase, antioxidant and anti-inflammatory potential.
枸杞小檗（小檗科）传统上用于治疗糖尿病。本研究旨在确定水提取物和甲醇提取物对全株植物的抗氧化、抗糖尿病和抗炎作用。采用福林-酚试剂法测定总酚含量，采用2,2-二苯基-1-苦基肼（DPPH）法测定抗氧化活性。采用α淀粉酶测定法测定体外抗糖尿病活性。对血糖正常的大鼠进行急性降血糖活性研究。对四氧嘧啶诱导的糖尿病大鼠进行14天的亚急性抗糖尿病作用研究。甲醇提取物的总酚含量为183.5±1mg/g没食子酸当量（GAE）。在抗氧化和α淀粉酶抑制试验中，甲醇提取物的IC50分别为242μg/mL和37.26mg/mL。给血糖正常的大鼠灌胃甲醇提取物后，在90分钟和120分钟时显示出显著的降血糖作用。甲醇提取物（500mg/kg提取物）在第14天显著降低血糖。甲醇提取物（500mg/kg）显著降低糖尿病大鼠肿瘤坏死因子（TNF-α）和白细胞介素（IL-6）的浓度，同时降低总胆固醇和甘油三酯水平。给予甲醇提取物还改善了肝脏指标。该研究表明，甲醇提取物具有抗糖尿病作用，这可能归因于其α淀粉酶、抗氧化和抗炎潜力。
