Xie Jia, Yang Xiaohan, Duan Lei, Chen Keyi, Liu Pan, Zhan Wenli, Zhang Changbin, Zhao Hongyu, Wei Mengru, Tang Yuan, Luo Mingyong
Medical Genetic Centre, Guangdong Women and Children's Hospital, Guangzhou Medical University, Guangzhou, China.
Medical Genetic Centre, Guangdong Women and Children Hospital, Guangzhou, China.
Front Microbiol. 2021 Feb 4;12:629533. doi: 10.3389/fmicb.2021.629533. eCollection 2021.
Hand, foot, and mouth disease (HFMD) is a common infectious disease affecting mainly children under 5 years of age. Coxsackievirus A6 (CVA-6), a major causative pathogen of HFMD, has caused outbreaks in recent years. Currently, no effective vaccine or antiviral treatments are available. In this study, one-step reverse-transcription recombinase polymerase amplification (RT-RPA), combined with a disposable lateral flow strip (LFS) assay, was developed to detect CVA-6. This assay can be performed in less than 35 min at 37°C without expensive instruments, and the result can be observed directly with the naked eye. The sensitivity of the RT-RPA-LFS was 10 copies per reaction, which was comparable to that of the conventional real-time quantitative polymerase chain reaction (qPCR) assays. Moreover, the assay specificity was 100%. The clinical performance of the RT-RPA-LFS assay was evaluated using 142 clinical samples, and the coincidence rate between RT-RPA-LFS and qPCR was 100%. Therefore, our RT-RPA-LFS assay provides a simple and rapid approach for point-of-care CVA-6 diagnosis.
手足口病（HFMD）是一种常见的传染病，主要影响5岁以下儿童。柯萨奇病毒A6（CVA-6）是手足口病的主要致病病原体，近年来已引发多起疫情。目前，尚无有效的疫苗或抗病毒治疗方法。在本研究中，开发了一种一步法逆转录重组酶聚合酶扩增（RT-RPA）技术，并结合一次性侧向流动试纸条（LFS）检测法来检测CVA-6。该检测方法可在37°C下35分钟内完成，无需昂贵仪器，结果可直接用肉眼观察。RT-RPA-LFS的灵敏度为每个反应10个拷贝，与传统实时定量聚合酶链反应（qPCR）检测方法相当。此外，该检测方法的特异性为100%。使用142份临床样本评估了RT-RPA-LFS检测方法的临床性能，RT-RPA-LFS与qPCR的符合率为100%。因此，我们的RT-RPA-LFS检测方法为床旁快速诊断CVA-6提供了一种简单、快速的方法。
