Nozari Masoumeh, Nahavandi Arezo, Zeinivand Motahareh, Eslami Gharaati Maryam, Godarzi Mina, Ahmadi Mohammad, Jamali-Raeufy Nida
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Department of Neuroscience, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Basic Clin Neurosci. 2020 Jul-Aug;11(4):413-422. doi: 10.32598/bcn.11.4.1775.2. Epub 2020 Jul 1.
Stress predisposes organisms to depression and cognitive impairments, and seems to interact with metabolic homeostasis. The inflammatory response and the upregulation of proinflammatory cytokines are some of the consequences related to chronic stress. In this study, we investigated the preventive effect of chronic administration of ibuprofen, as an inhibitor of cyclooxygenases, on the cognitive and behavioral alterations and the weight gain reduction induced by simultaneous chronic restraint stress in rats.
Male Wistar rats were subjected to chronic restraint stress and injected daily with the variable doses of ibuprofen or vehicle, for 21 consecutive days. Then, all animals were tested with the forced swim test and passive avoidance conditioning. Also, the weight of the animals was recorded before and after the interventions. Ultimately, plasma interleukin 6 (IL-6) levels were measured.
Chronic stress increased depressive-like behaviors, impaired learning, and disrupted the normal weight gain. However, the animals that received the highest dose of ibuprofen showed less depressive-like behaviors, a better avoidance memory, and a higher weight gain. However, the level of plasma IL-6 did not differ significantly between the study groups.
The administration of ibuprofen prevents the cognitive and behavioral consequences of chronic stress. During the recovery, the plasma levels of IL-6 were not elevated by stress, and the IL-6 levels did not predict the behavioral performance of the stressed animals. The exact mechanisms of the protective effects of ibuprofen against chronic stress need to be further investigated.
应激使生物体易患抑郁症和认知障碍，且似乎与代谢稳态相互作用。炎症反应和促炎细胞因子的上调是与慢性应激相关的一些后果。在本研究中，我们研究了作为环氧化酶抑制剂的布洛芬长期给药对大鼠因同时遭受慢性束缚应激所诱导的认知和行为改变以及体重增加减少的预防作用。
雄性Wistar大鼠遭受慢性束缚应激，并连续21天每天注射不同剂量的布洛芬或赋形剂。然后，所有动物均接受强迫游泳试验和被动回避条件反射测试。此外，在干预前后记录动物的体重。最后，测量血浆白细胞介素6（IL-6）水平。
慢性应激增加了抑郁样行为，损害了学习能力，并扰乱了正常的体重增加。然而，接受最高剂量布洛芬的动物表现出较少的抑郁样行为、更好的回避记忆和更高的体重增加。然而，研究组之间血浆IL-6水平无显著差异。
布洛芬给药可预防慢性应激的认知和行为后果。在恢复过程中，应激并未使血浆IL-6水平升高，且IL-6水平无法预测应激动物的行为表现。布洛芬对慢性应激的保护作用的确切机制需要进一步研究。
