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使用芯片实验室进行物种鉴定的现场基因分析。

On-site genetic analysis for species identification using lab-on-a-chip.

作者信息

Wimbles Ryan, Melling Louise M, Cain Bradley, Davies Naomi, Doherty Jason, Johnson Bridget, Shaw Kirsty J

机构信息

Department of Natural Sciences Manchester Metropolitan University Manchester UK.

Knowsley Safari Prescot UK.

出版信息

Ecol Evol. 2021 Jan 29;11(4):1535-1543. doi: 10.1002/ece3.7053. eCollection 2021 Feb.

DOI:10.1002/ece3.7053
PMID:33613987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7882957/
Abstract

This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (). The development of a microfluidic device, which can be used in the field, offers a portable and cost-effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit-based methods within just 5 minutes. The use of a color-changing loop-mediated isothermal amplification reaction for simultaneous detection of the cytochrome B sequence of enabled positive results to be obtained within as little as 30 minutes. Field testing was performed at Knowsley Safari to demonstrate real-world applicability of the microfluidic device for testing of biological samples.

摘要

本文介绍了一种能够对粪便样本进行基因分析以识别白犀牛的微流控装置。这种可在野外使用的微流控装置的开发，为DNA分析和物种识别提供了一种便携且经济高效的解决方案，有助于保护工作。与传统的基于试剂盒的方法相比，DNA提取过程的优化在短短5分钟内就产生了相当的产量。使用变色环介导的等温扩增反应同时检测细胞色素B序列，能在短短30分钟内获得阳性结果。在诺思利野生动物园进行了现场测试，以证明该微流控装置在生物样本检测中的实际适用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/315060527b62/ECE3-11-1535-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/90120a9d934b/ECE3-11-1535-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/d316bec0e51d/ECE3-11-1535-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/2d9b0a74b17c/ECE3-11-1535-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/0e0077f36c79/ECE3-11-1535-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/fa360c18fd8a/ECE3-11-1535-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/315060527b62/ECE3-11-1535-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/90120a9d934b/ECE3-11-1535-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/d316bec0e51d/ECE3-11-1535-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/2d9b0a74b17c/ECE3-11-1535-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/0e0077f36c79/ECE3-11-1535-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/fa360c18fd8a/ECE3-11-1535-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e139/7882957/315060527b62/ECE3-11-1535-g006.jpg

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