Investigation of neurons that express Dpr/DIP cell adhesion molecules.

reproductive behaviors are directed by neurons. A reanalysis of genomic studies shows that genes encoding and immunoglobulin superfamily (IgSF) members are expressed in neurons. We find that each and ( ∩ ) overlapping expression pattern is similar in both sexes, but there are dimorphisms in neuronal morphology and cell number. Behavioral studies of ∩ perturbation genotypes indicate that the mushroom body functions together with the lateral protocerebral complex to direct courtship behavior. A single-cell RNA-seq analysis of neurons shows that many have high expression in a small set of neurons, whereas the are often expressed in a larger set of neurons at intermediate levels, with a myriad of expression combinations. Functionally, we find that perturbations of sex hierarchy genes and of change the sex-specific morphologies of ∩ neurons.