Miller J A, Hoffer B J, Zahniser N R
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
J Neurosci Methods. 1988 Jan;22(3):233-8. doi: 10.1016/0165-0270(88)90044-1.
A mathematical model which accounts for the non-linear response of both the camera and film in computer-aided quantitative autoradiography is derived. This model and the algorithm to fit the data for radioactive standards by a non-linear least-squares procedure to this model are described. The usefulness of this technique is demonstrated by employing it to analyze a set of 14C-labeled brain paste standards which were exposed to Ultrofilm in such a way that the full response range of the film was used. This technique can be readily implemented on most commercially available image analysis systems and is compatible with most types of video cameras.
推导了一种数学模型，该模型考虑了计算机辅助定量放射自显影中相机和胶片的非线性响应。描述了此模型以及通过非线性最小二乘法将放射性标准数据拟合到该模型的算法。通过用该技术分析一组暴露于紫外胶片的14C标记脑糊标准品来证明其有效性，这种方式使用了胶片的整个响应范围。该技术可以很容易地在大多数商用图像分析系统上实现，并且与大多数类型的摄像机兼容。
