Johnson E W, Sybirska E, al-Tikriti M, Innis R B
Department of Psychiatry, Yale University School of Medicine, West Haven, CT 06510.
Int J Rad Appl Instrum A. 1991;42(12):1199-201. doi: 10.1016/0883-2889(91)90197-9.
Autoradiographic images can be analyzed with computer-assisted microdensitometry relative to radioactive reference standards to provide quantitative measurements of regional radioactivity concentrations. [123I]Iodine containing sections of brain paste have been calibrated relative to plastic-embedded tritium(3H) and 125Iodine standards. For exposure times of 8, 12 and 24 h, plastic-embedded standards covered a range concentrations of [123I]iodine from 1000 to 100,000 dpm/mg wet weight. Iodine-123 radioactivity was linear with section thickness from 5 to 30 microns. These studies confirmed the feasibility of using commercially available longer-lived reference standards to provide [123I]iodine equivalent values in absolute units of dpm (or microCi) per mg tissue.
放射自显影图像可通过计算机辅助显微密度测定法相对于放射性参考标准进行分析，以提供区域放射性浓度的定量测量。含[123I]碘的脑糊切片已相对于塑料包埋的氚（3H）和125碘标准进行了校准。对于8、12和24小时的曝光时间，塑料包埋标准涵盖了[123I]碘浓度范围为1000至100,000 dpm/毫克湿重。碘-123放射性与5至30微米的切片厚度呈线性关系。这些研究证实了使用市售的寿命更长的参考标准以每毫克组织的dpm（或微居里）绝对单位提供[123I]碘当量值的可行性。
