Kirillova Anastasia, Bunyaeva Ekaterina, Van Ranst Heidi, Khabas Grigory, Farmakovskaya Maria, Kamaletdinov Nail, Nazarenko Tatiana, Abubakirov Aydar, Sukhikh Gennady, Smitz Johan E J
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after V.I.Kulakov, of the Ministry of Healthcare of Russian Federation, Moscow, Russia.
Follicle Biology Laboratory (FOBI), Vrije Universiteit Brussel, Brussels, Belgium.
J Assist Reprod Genet. 2021 Jun;38(6):1331-1340. doi: 10.1007/s10815-021-02118-z. Epub 2021 Feb 22.
To investigate the developmental competence of ovarian tissue oocytes from patients with gynecological tumors using a biphasic in vitro maturation system with capacitation (CAPA-IVM) in comparison with standard IVM.
This sibling pilot study included 210 oocytes in 10 patients with gynecological malignancies. After ovariectomies, ovaries were cut into even halves and immature cumulus-oocyte complexes (COCs) were retrieved from the ovarian tissue. COCs were separately cultured in either a biphasic CAPA-IVM system for 53 h or in standard IVM for 48 h. After IVM, all COCs were denuded and mature oocytes were either vitrified (N=5) or used for ICSI (N=5). Embryos were cultured for 5-6 days and obtained blastocysts were vitrified.
Use of the CAPA-IVM system led to a higher meiotic maturation rate in ovarian tissue oocytes (OTO) compared to standard IVM (56 vs 35%, p=0.0045) and had a tendency to result in lower degeneration after IVM. Only the CAPA-IVM method supported blastocyst formation.
The biphasic in vitro maturation system improved the competence of OTO in comparison to the standard IVM method. The study suggests that fertility preservation programs could become more efficient using IVM after capacitation culture.
使用具有获能功能的双相体外成熟系统（CAPA-IVM），与标准体外成熟（IVM）方法相比较，研究妇科肿瘤患者卵巢组织卵母细胞的发育能力。
这项同胞对照初步研究纳入了10例妇科恶性肿瘤患者的210枚卵母细胞。卵巢切除术后，将卵巢切成均匀的两半，从卵巢组织中获取未成熟的卵丘-卵母细胞复合体（COC）。COC分别在双相CAPA-IVM系统中培养53小时或在标准IVM系统中培养48小时。体外成熟后，所有COC去除颗粒细胞，成熟卵母细胞要么进行玻璃化冷冻（N = 5），要么用于卵胞浆内单精子注射（ICSI）（N = 5）。胚胎培养5 - 6天，获得的囊胚进行玻璃化冷冻。
与标准IVM相比，使用CAPA-IVM系统可使卵巢组织卵母细胞（OTO）的减数分裂成熟率更高（56%对35%，p = 0.0045），并且体外成熟后有较低退化率的趋势。只有CAPA-IVM方法支持囊胚形成。
与标准IVM方法相比，双相体外成熟系统提高了OTO的发育能力。该研究表明，获能培养后的体外成熟技术可提高生育力保存方案的效率。
