急性三甲基锡暴露诱导的大脑雌激素代谢的性别特异性变化。
Sex-specific Changes in Brain Estrogen Metabolism Induced by Acute Trimethyltin Exposure.
作者信息
Lee Jung Ho, Cho Sung-Hee, Jang Eun Hye, Kim Soon Ae
机构信息
Department of Pharmacology, School of Medicine, Eulji University, Daejeon, Republic of Korea.
Chemical Analysis Center, Korea Research Institute of Chemical Technology (KRICT), Daejeon, Republic of Korea.
出版信息
In Vivo. 2021 Mar-Apr;35(2):793-797. doi: 10.21873/invivo.12319.
BACKGROUND/AIM: In this study, we investigated sex-specific effects of acute exposure to trimethyltin, a known neurotoxicant on metabolic steroids.
MATERIALS AND METHODS
We administered intraperitoneally 2.3 mg/kg trimethyltin to 4-week-old male mice and measured the levels of metabolic steroids 24 h after treatment. We also measured mRNA and protein levels of cytochrome P450 1B1 using real-time polymerase chain reaction and western blotting.
RESULTS
Cortisol levels in the cortex increased in both sexes following acute trimethyltin exposure. The estradiol levels decreased, and the 4-hydroxyestradiol levels increased only in females. We also observed increased cytochrome P450 1B1 mRNA and protein levels only in the female cortex.
CONCLUSION
Acute trimethyltin exposure induces distinct sex-specific metabolic changes in the brain before significant sexual maturation.
背景/目的：在本研究中，我们调查了急性暴露于三甲基锡（一种已知的对代谢类固醇有神经毒性的物质）的性别特异性影响。
材料与方法
我们给4周龄雄性小鼠腹腔注射2.3mg/kg三甲基锡，并在处理后24小时测量代谢类固醇的水平。我们还使用实时聚合酶链反应和蛋白质印迹法测量细胞色素P450 1B1的mRNA和蛋白质水平。
结果
急性暴露于三甲基锡后，两性皮质中的皮质醇水平均升高。雌二醇水平降低，而仅在雌性中4-羟基雌二醇水平升高。我们还仅在雌性皮质中观察到细胞色素P450 1B1的mRNA和蛋白质水平增加。
结论
在明显的性成熟之前，急性暴露于三甲基锡会在大脑中诱导明显的性别特异性代谢变化。
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