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静电场对骨愈合过程影响的研究:Wistar-白化雄性大鼠胫骨骨折实验模型研究

Investigation into the effects of static and electric fields on bone healing process: An experimental tibial fracture model study in Wistar-Albino male rats.

作者信息

Aslan Ahmet, Kocak Ahmet, Comlekci Selcuk, Kirdemir Vecihi

机构信息

Department of Orthopaedics and Traumatology, Alanya Alaaddin Keykubat University Faculty of Medicine, Antalya, Turkey.

Department of Histology and Embriology, Kutahya Health Science University Faculty of Medicine, Kutahya, Turkey.

出版信息

North Clin Istanb. 2020 Nov 23;8(1):8-14. doi: 10.14744/nci.2020.04764. eCollection 2021.


DOI:10.14744/nci.2020.04764
PMID:33623867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7881430/
Abstract

OBJECTIVE: In this experimental study, we aimed to investigate whether 0 Hz-Static and 50 Hz-Electric fields have an effect on bone healing. METHODS: In this study, 45 male Wistar-Albino rats were equally and randomly separated into three groups as follows: a 0 Hz-Static electric field (SEF), a 50-Hz low-frequency electric field (LFEF) and a control group. A manual fracture was performed in the left tibia diaphysis of all rats, and fractures were fixed using circular plaster over the knee. The LFEF group was exposed to 50 Hz electric field for 30 minutes a day, five days a week, for a total of eight weeks. The SEF group was exposed to 0 Hz electric field within the same time interval. The control group was held in identical environmental conditions, without exposure to electric field. Periodic radiographs were taken from all the animals. At the end of this study, rats were sacrificed and mechanical/histopathologic examinations were performed. RESULTS: Radiologic, mechanical and histologic scores of the LFEF group were lower than those of the SEF and control groups; however, no significant difference was found in group comparisons in terms of average histologic and radiologic scores (p>0.05). CONCLUSION: Results extracted from the current study suggest that 0-hz static and 50-hz electric field exposures affect bone healing tissue of tibial fracture models in rats, although it is not significant.

摘要

目的:在本实验研究中,我们旨在探究0 Hz静电磁场和50 Hz电场是否对骨折愈合有影响。 方法:在本研究中,45只雄性Wistar - 白化大鼠被平均随机分为三组,如下:0 Hz静电磁场(SEF)组、50 Hz低频电场(LFEF)组和对照组。对所有大鼠的左胫骨干中段进行手法骨折,并使用环形石膏固定膝关节上方的骨折部位。LFEF组每天暴露于50 Hz电场30分钟,每周5天,共8周。SEF组在相同时间间隔内暴露于0 Hz电场。对照组置于相同环境条件下,不暴露于电场。定期对所有动物进行X线摄影。在本研究结束时,处死大鼠并进行力学/组织病理学检查。 结果:LFEF组的放射学、力学和组织学评分低于SEF组和对照组;然而,在平均组织学和放射学评分的组间比较中未发现显著差异(p>0.05)。 结论:本研究结果表明,0 Hz静电磁场和50 Hz电场暴露虽对大鼠胫骨骨折模型的骨折愈合组织有影响,但不显著。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/cb2a991c320f/NCI-8-8-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/3019c5d907b6/NCI-8-8-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/70933f76cb13/NCI-8-8-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/9bd3fd666834/NCI-8-8-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/7a690c6d0b17/NCI-8-8-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/d46f763a793e/NCI-8-8-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/cb2a991c320f/NCI-8-8-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/3019c5d907b6/NCI-8-8-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/70933f76cb13/NCI-8-8-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/9bd3fd666834/NCI-8-8-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/7a690c6d0b17/NCI-8-8-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/d46f763a793e/NCI-8-8-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f494/7881430/cb2a991c320f/NCI-8-8-g006.jpg

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引用本文的文献

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The treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect.

Front Bioeng Biotechnol. 2024-7-2

本文引用的文献

[1]
The Impact of the Low Frequency of the Electromagnetic Field on Human.

Adv Exp Med Biol. 2020

[2]
Effects of static magnetic fields on bone microstructure and mechanical properties in mice.

Electromagn Biol Med. 2018

[3]
Effects of local vibration and pulsed electromagnetic field on bone fracture: A comparative study.

Bioelectromagnetics. 2017-7

[4]
Pulsed electromagnetic fields promote osteogenesis and osseointegration of porous titanium implants in bone defect repair through a Wnt/β-catenin signaling-associated mechanism.

Sci Rep. 2016-8-24

[5]
Efficacy of Electrical Stimulators for Bone Healing: A Meta-Analysis of Randomized Sham-Controlled Trials.

Sci Rep. 2016-8-19

[6]
The effect of prenatal exposure to 1800 MHz electromagnetic field on calcineurin and bone development in rats.

Acta Cir Bras. 2016-2

[7]
Fracture healing: mechanisms and interventions.

Nat Rev Rheumatol. 2015-1

[8]
The effects of low-intensity pulsed ultrasound and pulsed electromagnetic fields bone growth stimulation in acute fractures: a systematic review and meta-analysis of randomized controlled trials.

Arch Orthop Trauma Surg. 2014-6-4

[9]
Pulsed electromagnetic field treatment enhances healing callus biomechanical properties in an animal model of osteoporotic fracture.

Bioelectromagnetics. 2014-9

[10]
A novel single pulsed electromagnetic field stimulates osteogenesis of bone marrow mesenchymal stem cells and bone repair.

PLoS One. 2014-3-14

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