Zhou Jing, Yang Dan, Liu Guohui, Li Siying, Feng Wennan, Yang Guocheng, He Jin, Shan Yuping
School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China.
Analyst. 2021 Apr 7;146(7):2321-2329. doi: 10.1039/d1an00220a. Epub 2021 Feb 24.
Guanine (G) oxidation products, such as 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-oxo-guanine (8-OXOG), have been widely studied as promising biomarkers for DNA oxidative damage. In this work, we develop a new method to detect G oxidative products released from live cells after chromium (vi) ion or hydrogen peroxide treatments by using a glass nanopipette-based flexible gold nanoelectrode (fGNE). Specific response to G oxidative products with high sensitivity can be detected from the fGNE tip through integrated electrochemical measurements and surface-enhanced Raman spectroscopy. The fGNE apex can be positioned very close to the cell membrane noninvasively because of its high flexibility and nanoscale tip size. With the assistance of the electrophoretic force, the fGNEs can effectively collect and detect the G-derived DNA damage products released from individual cells in the cell culture medium with high sensitivity.
鸟嘌呤（G）氧化产物，如8-羟基-2'-脱氧鸟苷（8-OHdG）和8-氧代鸟嘌呤（8-OXOG），作为DNA氧化损伤的潜在生物标志物已被广泛研究。在这项工作中，我们开发了一种新方法，通过使用基于玻璃纳米移液器的柔性金纳米电极（fGNE）来检测六价铬离子或过氧化氢处理后活细胞释放的G氧化产物。通过集成电化学测量和表面增强拉曼光谱，可以从fGNE尖端检测到对G氧化产物的高灵敏度特异性响应。由于其高柔韧性和纳米级尖端尺寸，fGNE顶点可以非常接近细胞膜非侵入性地定位。在电泳力的帮助下，fGNE可以有效地收集和检测细胞培养基中单个细胞释放的G衍生DNA损伤产物，具有高灵敏度。
